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One daughter is maintained as a GSC<\/jats:styled-content>, whereas the other initiates cyst formation, a process involving four synchronous mitotic divisions that form 2\u2010, 4\u2010, 8\u2010, and 16\u2010cell cysts. In this study, we found that reduction in the level of NHP<\/jats:styled-content>2,<\/jats:italic> a component of the H\/ACA<\/jats:styled-content> small nucleolar ribonucleoprotein complex that catalyzes rRNA<\/jats:styled-content> pseudouridylation, promotes progression to 8\u2010cell cysts. NHP<\/jats:styled-content>2 protein was concentrated in the nucleoli of germline cells during cyst formation. NHP<\/jats:styled-content>2 expression, as well as the nucleolar size, abruptly decreased during progression from 2\u2010cell to 4\u2010cell cysts. Reduction in NHP<\/jats:styled-content>2<\/jats:italic> activity in the germline caused accumulation of 4\u2010 and 8\u2010cell cysts and decreased the number of single cells. In addition, NHP<\/jats:styled-content>2<\/jats:italic> knockdown impaired the transition to 16\u2010cell cysts. Furthermore, a tumorous phenotype caused by Sex\u2010lethal<\/jats:italic> (Sxl<\/jats:italic>) knockdown, which is characterized by accumulation of single and two\u2010cell cysts, was partially rescued by NHP<\/jats:styled-content>2<\/jats:italic> knockdown. When Sxl<\/jats:italic> and NHP<\/jats:styled-content>2<\/jats:italic> activities were concomitantly repressed, the numbers of four\u2010 and eight\u2010cell cysts were increased. In addition, Sxl protein physically interacted with NHP<\/jats:styled-content>2 <\/jats:italic>mRNA<\/jats:styled-content> in ovaries. Thus, it is reasonable to conclude that Sxl<\/jats:italic> represses NHP<\/jats:styled-content>2<\/jats:italic> activity at the post\u2010transcriptional level to promote proper cyst formation. Because NHP<\/jats:styled-content>2<\/jats:italic> knockdown did not affect global protein synthesis in the germarium, we speculate that changes in NHP<\/jats:styled-content>2\u2010dependent pseudouridylation, which is involved in translation of specific mRNA<\/jats:styled-content>s, must be intact in order to promote proper cyst formation.<\/jats:p>","DOI":"10.1111\/dgd.12539","type":"journal-article","created":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T06:56:37Z","timestamp":1527663397000},"page":"248-259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Downregulation of NHP<\/scp>2 promotes proper cyst formation in Drosophila<\/i> ovary"],"prefix":"10.1111","volume":"60","author":[{"given":"Shumpei","family":"Morita","sequence":"first","affiliation":[{"name":"Graduate School of Life and Environmental Sciences University of Tsukuba Tsukuba Ibaraki 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