{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T14:27:26Z","timestamp":1774103246548,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T00:00:00Z","timestamp":1556496000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"China National Health and Family Planning Commission","award":["201402007"],"award-info":[{"award-number":["201402007"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dev Growth Differ"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hirschsprung's disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>) is characterized by missing of enteric neurons in the terminal areas of the whole gut, which is causally related to poor proliferation of enteric neural crest cells (<jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s). Our aim is to explore how miR\u2010431\u20105p interacts with its target gene in regulation of proliferation of <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mouse model of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> was established by Benzalkonium chloride (<jats:styled-content style=\"fixed-case\">BAC<\/jats:styled-content>) treatment. Quantitative Real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and western blotting were performed to determine the miR\u2010431\u20105p and the <jats:styled-content style=\"fixed-case\">LRSAM<\/jats:styled-content>1 expression in colon tissues of the <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> group (<jats:italic>n<\/jats:italic>\u00a0=\u00a08) and the control group (<jats:italic>n<\/jats:italic>\u00a0=\u00a08) and in <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s isolated from colon tissues. <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>\u20108 assay was performed to detect the proliferation of <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s after transfection with miR\u2010431\u20105p mimics or miR\u2010431\u20105p inhibitor. Luciferase reporter assay was conducted to clarify the connections between miR\u2010431\u20105p and <jats:styled-content style=\"fixed-case\">LRSAM<\/jats:styled-content>1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Upregulation of miR\u2010431\u20105p and downregulation of <jats:styled-content style=\"fixed-case\">LRSAM<\/jats:styled-content>1 were found in <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. Downregulation of miR\u2010431\u20105p could promote cell proliferation of <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">LRSAM<\/jats:styled-content>1 was proved to be the target gene of miR\u2010431\u20105p by luciferase reporter assay. Moreover, proliferation of <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s was increased in the miR\u2010431\u20105p inhibitor group and was suppressed after knocking down <jats:styled-content style=\"fixed-case\">LRSAM<\/jats:styled-content>1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Downregulation of miR\u2010431\u20105p promoted proliferation of <jats:styled-content style=\"fixed-case\">ENCC<\/jats:styled-content>s via targeting <jats:styled-content style=\"fixed-case\">LRSAM<\/jats:styled-content>1, which provides an innovative and candidate target for treatment of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/dgd.12606","type":"journal-article","created":{"date-parts":[[2019,5,1]],"date-time":"2019-05-01T01:41:44Z","timestamp":1556674904000},"page":"294-302","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Downregulation of micro<scp>RNA<\/scp>\u2010431\u20105p promotes enteric neural crest cell proliferation via targeting <scp>LRSAM<\/scp>1 in Hirschsprung's disease"],"prefix":"10.1111","volume":"61","author":[{"given":"Bo","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Tianjin Medical University General Hospital Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of General Surgery Tianjin Union Medical Center Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6669-4007","authenticated-orcid":false,"given":"Xiao\u2010ye","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Tianjin Medical University General Hospital Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Surgery Capital Institute of Pediatrics Children's Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.017418"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cge.12032"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2007.10.067"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.010942"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00857"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","unstructured":"Bottai D. 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