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In order to understand region\u2010specific roles of Hey2 during cardiac morphogenesis, we generated <jats:italic>Hey2<\/jats:italic> conditional knockout (cKO) mice using <jats:italic>Mef2c\u2010AHF\u2010Cre<\/jats:italic>, which was active in the anterior part of the second heart field and the right ventricle and outflow tract of the heart. <jats:italic>Hey2<\/jats:italic> cKO neonates reproduced three anomalies commonly observed in <jats:italic>Hey2<\/jats:italic> null mice. An earliest morphological defect was the lack of right ventricular extension along the apico\u2010basal axis at midgestational stages. Underdevelopment of the right ventricle was present in all cKO neonates including those without apparent atresia of right\u2010sided atrioventricular connection. RNA sequencing analysis of cKO embryos identified that the gene expression of a non\u2010chamber T\u2010box factor Tbx2 was ectopically induced in the chamber myocardium of the right ventricle. Consistently, mRNA expression of the Mycn transcription factor, which was a cell cycle regulator transcriptionally repressed by Tbx2, was down regulated, and the number of S\u2010phase cells was significantly decreased in the right ventricle of cKO heart. These results suggest that Hey2 plays an important role in right ventricle development during cardiac morphogenesis, at least in part, through mitigating Tbx2\u2010dependent inhibition of <jats:italic>Mycn<\/jats:italic> expression.<\/jats:p>","DOI":"10.1111\/dgd.12707","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T13:49:39Z","timestamp":1610286579000},"page":"82-92","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A role of Hey2 transcription factor for right ventricle development through regulation of Tbx2\u2010Mycn pathway during cardiac morphogenesis"],"prefix":"10.1111","volume":"63","author":[{"given":"Daiki","family":"Seya","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology National Cerebral and Cardiovascular Center Research Institute Suita Osaka Japan"},{"name":"Department of Cell Physiology The Jikei University School of Medicine 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