{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T08:30:35Z","timestamp":1777451435993,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T00:00:00Z","timestamp":1686096000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["22H0279600"],"award-info":[{"award-number":["22H0279600"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["JP19K07268"],"award-info":[{"award-number":["JP19K07268"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dev Growth Differ"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although the heart is one of the most important organs for animal survival, its regenerative capacity varies among animal species. Notably, adult mammals cannot regenerate their hearts after damage such as acute myocardial infarction. In contrast, some vertebrate animals can regenerate the heart throughout their lives. Cross\u2010species comparative studies are important to understand the full picture of cardiac regeneration in vertebrates. Among the animal species able to regenerate the heart, some urodele amphibians, such as newts, possess a remarkable capacity for this process. Standardized methods of inducing cardiac regeneration in the newt are needed as a platform for studies comparing newts and other animal models. The procedures presented here describe amputation and cryo\u2010injury techniques for the induction of cardiac regeneration in <jats:italic>Pleurodeles waltl<\/jats:italic>, an emerging model newt species. Both procedures consist of simplified steps that require no special equipment. We additionally show some examples of the regenerative process obtained using these procedures. This protocol has been developed for <jats:italic>P. waltl<\/jats:italic>. However, these methods are also expected to be applicable to other newt and salamander species, facilitating comparative research with other model animals.<\/jats:p>","DOI":"10.1111\/dgd.12854","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T17:01:40Z","timestamp":1683565300000},"page":"266-271","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Step\u2010by\u2010step protocol for alternative injury models in newt cardiac regeneration"],"prefix":"10.1111","volume":"65","author":[{"given":"Hiromi","family":"Ikuta","sequence":"first","affiliation":[{"name":"Graduate school of Integrated Sciences for Life Hiroshima University  Hiroshima Japan"},{"name":"Amphibian Research Center Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Uemasu","sequence":"additional","affiliation":[{"name":"Division of Pediatrics and Perinatology, School of Medicine, Faculty of Medicine Tottori University  Tottori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuki","family":"Kyakuno","sequence":"additional","affiliation":[{"name":"Graduate school of Integrated Sciences for Life Hiroshima University  Hiroshima Japan"},{"name":"Amphibian Research Center Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Satoh","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, School of Life Sciences, Faculty of Medicine Tottori University  Tottori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyuki","family":"Namba","sequence":"additional","affiliation":[{"name":"Division of Pediatrics and Perinatology, School of Medicine, Faculty of Medicine Tottori University  Tottori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Division of Developmental Biology, School of Life Sciences, Faculty of Medicine Tottori University  Tottori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3676-5710","authenticated-orcid":false,"given":"Toshinori","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Graduate school of Integrated Sciences for Life Hiroshima University  Hiroshima Japan"},{"name":"Amphibian Research Center Hiroshima University  Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/248145a0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202000054"},{"key":"e_1_2_8_4_1","article-title":"Cryo\u2010injury induced heart regeneration in the axolotl and echocardiography and unbiased quantitative histology to evaluate regenerative progression","volume":"171","author":"Dittrich A.","year":"2021","journal-title":"J Vis Exp"},{"key":"e_1_2_8_5_1","article-title":"2D and 3D echocardiography in the axolotl (Ambystoma Mexicanum)","volume":"414","author":"Dittrich A.","year":"2018","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01964-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-022-00902-2"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11936-013-0288-8"},{"key":"e_1_2_8_9_1","first-page":"22","article-title":"Heart regeneration in the salamander relies on macrophage\u2010mediated control of fibroblast activation and the extracellular landscape. 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