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Damage to these axons leads to loss of vision. Despite research on neural circuit regeneration through transplantation, achieving precise axon projection remains challenging. To achieve optic nerve regeneration by transplantation, we employed the <jats:italic>Drosophila<\/jats:italic> visual system. We previously established a transplantation method for <jats:italic>Drosophila<\/jats:italic> utilizing photoreceptor precursor cells extracted from the eye disc. However, little axonal elongation of transplanted cells into the brain, the lamina, was observed. We verified axonal elongation to the lamina by modifying the selection process for transplanted cells. Moreover, we focused on N\u2010cadherin (Ncad), a cell adhesion factor, and Twinstar (Tsr), which has been shown to promote actin reorganization and induce axon elongation in damaged nerves. Overexpression of <jats:italic>Ncad<\/jats:italic> and <jats:italic>tsr<\/jats:italic> promoted axon elongation to the lamina, along with presynaptic structure formation in the elongating axons. Furthermore, overexpression of <jats:italic>Neurexin\u20101<\/jats:italic> (<jats:italic>Nrx\u20101<\/jats:italic>), encoding a protein identified as a synaptic organizer, was found to not only promote presynapse formation but also enhance axon elongation. By introducing <jats:italic>Ncad<\/jats:italic>, <jats:italic>tsr<\/jats:italic>, and <jats:italic>Nrx\u20101<\/jats:italic>, we not only successfully achieved axonal projection of transplanted cells to the brain beyond the retina, but also confirmed the projection of transplanted cells into a deeper ganglion, the medulla. The present study offers valuable insights to realize regeneration through transplantation in a more complex nervous system.<\/jats:p>","DOI":"10.1111\/dgd.12916","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T01:05:50Z","timestamp":1708909550000},"page":"205-218","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cell adhesion and actin dynamics factors promote axonal extension and synapse formation in transplanted <i>Drosophila<\/i> photoreceptor cells"],"prefix":"10.1111","volume":"66","author":[{"given":"Riku","family":"Iwanaga","sequence":"first","affiliation":[{"name":"School of Life Science and Technology, Tokyo Institute of Technology  Yokahama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nagisa","family":"Yahagi","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology, Tokyo Institute of Technology  Yokahama 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