{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:57:53Z","timestamp":1747184273948,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T00:00:00Z","timestamp":1712707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dev Growth Differ"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The northern house gecko <jats:italic>Hemidactylus flaviviridis<\/jats:italic> exhibits appendage\u2010specific responses to injuries. The autotomized tail regenerates, whereas the severed limb fails to regrow. Many site\u2010specific cellular processes influence tail regeneration. Herein, we analyzed the epithelial\u2013mesenchymal transition contrast in the lizard's amputated appendages (tail and limb). Morphological observations in the healing frame indicated the formation of regeneration blastema in the tail and scar formation in limb. Histology of the tail showed that epithelial cells closer to mesenchyme appeared less columnar and loosely packed, with little intercellular matrix. Whereas in the limb, the columnar epithelial cells remained tightly packed. Collagen deposition was seen in the limb at the intersection of wound epithelium and mesenchyme, favoring scarring by blocking the epithelial\u2013mesenchymal transition. Markers for epithelial\u2013mesenchymal transition were assessed at transcript and protein levels. The regenerating tail showed upregulation of N\u2010cadherin, vimentin, and PCNA, favoring epithelial\u2013mesenchymal transition, cell migration, and proliferation, respectively. In contrast, the scarring limb showed persistently elevated levels of E\u2010cadherin and EpCAM, indicating retention of epithelial characteristics. An attempt was made to screen the resident epithelial stem cell population in both appendages to check their potential role in the epithelial\u2013mesenchymal transition (EMT), hence the differential wound healing. Upregulation in transcript and protein levels of Nanog and Sox2 was observed in the regenerating tail. Fluorescence\u2010activated cell sorting (FACS) provided supporting evidence that the epithelial stem cell population in tail remained significantly higher than in limb. Thus, this study focuses on the mechanistic role of the epithelial\u2013mesenchymal transition in wound healing, highlighting the molecular details of regeneration and scarring events.<\/jats:p>","DOI":"10.1111\/dgd.12923","type":"journal-article","created":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T02:56:10Z","timestamp":1712804170000},"page":"285-296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Epithelial\u2013mesenchymal transition contrast in the amputated tail and limb of the northern house gecko, <scp><i>Hemidactylus flaviviridis<\/i><\/scp>"],"prefix":"10.1111","volume":"66","author":[{"given":"Pooja","family":"Raval","sequence":"first","affiliation":[{"name":"Department of Zoology, Faculty of Science The Maharaja Sayajirao University of Baroda  Vadodara India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kashmira","family":"Khaire","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science The Maharaja Sayajirao University of Baroda  Vadodara India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shashikant","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science The Maharaja Sayajirao University of Baroda  Vadodara India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6559-022X","authenticated-orcid":false,"given":"Suresh","family":"Balakrishnan","sequence":"additional","affiliation":[{"name":"Department of Zoology, Faculty of Science The Maharaja Sayajirao University of Baroda  Vadodara India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,10]]},"reference":[{"issue":"19","key":"e_1_2_8_2_1","first-page":"3440","article-title":"Epithelial\u2010mesenchymal transition transcription factors control pluripotent adult stem cell migration in vivo in planarians","volume":"144","author":"Abnave P.","year":"2017","journal-title":"Development"},{"volume-title":"Morphological and cellular aspects of tail and limb regeneration in lizards: A model system with implications for tissue regeneration in mammals","year":"2009","author":"Alibardi L.","key":"e_1_2_8_3_1"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1111\/j.1463-6395.2009.00409.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1002\/dvdy.178"},{"issue":"5","key":"e_1_2_8_6_1","first-page":"301","article-title":"Autotomy and regeneration in reptiles","volume":"15","author":"Bellairs D. 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