{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:11:22Z","timestamp":1792458682071,"version":"4.5.0"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T00:00:00Z","timestamp":1735171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Development, Growth &amp; Differentiation"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Transcription factors collaborate with epigenetic regulatory factors to orchestrate cardiac differentiation for heart development, but the underlying mechanism is not fully understood. Here, we report that GATA\u20106 induces cardiac differentiation but peroxisome proliferator\u2010activated receptor \u03b1 (PPAR\u03b1) reverses GATA\u20106\u2010induced cardiac differentiation, possibly because GATA\u20106\/PPAR\u03b1 recruits the polycomb protein complex containing EZH2\/Ring1b\/BMI1 to the promoter of the cardiac\u2010specific \u03b1\u2010myosin heavy chain (\u03b1\u2010MHC) gene and suppresses \u03b1\u2010MHC expression, which ultimately inhibits cardiac differentiation. Furthermore, Ring1b ubiquitylates PPAR\u03b1 and GATA\u20106. By overexpression and knockout of EZH2\/BMI1, it was demonstrated that the polycomb protein complex inhibits cardiac differentiation induced by GATA\u20106 and PPAR\u03b1. Together, our results demonstrate that the polycomb protein complex interacts with GATA\u20106\/PPAR\u03b1 to inhibit cardiac differentiation, a finding that could facilitate the development of new therapies for congenital heart disease.<\/jats:p>","DOI":"10.1111\/dgd.12953","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T04:47:18Z","timestamp":1737694038000},"page":"23-32","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The polycomb protein complex interacts with <scp>GATA<\/scp>\u20106\/<scp>PPAR\u03b1<\/scp> to inhibit \u03b1\u2010<scp>MHC<\/scp> expression"],"prefix":"10.1111","volume":"67","author":[{"given":"Fei\u2010Fei","family":"Dai","sequence":"first","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology, School of Basic Medical Sciences Zhengzhou University  Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; 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