{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T01:40:59Z","timestamp":1776994859384,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25114003"],"award-info":[{"award-number":["25114003"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Development, Growth &amp;amp; Differentiation"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Germ cells in zebrafish are specified by a preformation mechanism, unlike in mice. Max (Myc associated factor X) and its associated PRC1.6 (polycomb repressive complex 1.6) act as negative regulators of germ cell\u2010 and meiosis\u2010related genes in mice; however, their function in zebrafish remains unknown. Here, we generated\n                    <jats:italic>max<\/jats:italic>\n                    mutants in zebrafish using genome\u2010editing techniques to investigate the role of Max in germ cell development and meiosis. No obvious morphological abnormalities were observed in the mutants up to 4\u2009days post fertilization (dpf), likely due to maternally supplied\n                    <jats:italic>max<\/jats:italic>\n                    mRNAs. However, the mutants failed to form an inflated swim bladder and gradually died after 10 dpf. The number of primordial germ cells (PGCs) did not differ between the\n                    <jats:italic>max<\/jats:italic>\n                    mutant and wild\u2010type larvae up to 12 dpf. In contrast, analysis of protein expression levels of germ cell\u2013related and meiosis\u2010related genes in PGCs at 9 dpf revealed that the levels of Sycp2, Sycp3, and Dmc1 proteins were significantly increased in\n                    <jats:italic>max<\/jats:italic>\n                    mutants, whereas the expression of Ddx4 and Piwil1 remained unchanged. Together, these results suggest that zebrafish Max selectively represses the expression of a subset of meiotic genes in PGCs.\n                  <\/jats:p>","DOI":"10.1111\/dgd.70053","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:21:14Z","timestamp":1776262874000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Derepression of a Subset of Meiotic Proteins in Primordial Germ Cells of\n                    <i>max<\/i>\n                    Mutant Zebrafish"],"prefix":"10.1111","volume":"68","author":[{"given":"Yuya","family":"Hasegawa","sequence":"first","affiliation":[{"name":"Department of Gene Function and Phenomics National Institute of Genetics  Mishima Japan"},{"name":"Fisheries Technology Institute Japan Fisheries Research and Education Agency  Goto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiro","family":"Kawasaki","sequence":"additional","affiliation":[{"name":"Department of Gene Function and Phenomics National Institute of Genetics  Mishima Japan"},{"name":"Research Organization of Science and Technology Ritsumeikan University  Kusatsu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of Gene Function and Phenomics National Institute of Genetics  Mishima Japan"},{"name":"Graduate School of Science and Engineering Saitama University  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7026-6355","authenticated-orcid":false,"given":"Yasuhisa","family":"Matsui","sequence":"additional","affiliation":[{"name":"Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer Tohoku University  Sendai Japan"},{"name":"University of Human Arts and Science  Saitama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8403-8281","authenticated-orcid":false,"given":"Noriyoshi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Gene Function and Phenomics National Institute of Genetics  Mishima Japan"},{"name":"Department of Genetics, School of Life Science SOKENDAI (The Graduate University for Advanced Studies)  Mishima Japan"},{"name":"Graduate School of Agriculture Kyoto University  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky379"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.112.147645"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-018-0650-z"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature24268"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2012.03.003"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1712408"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.21064"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.01.002"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2486-6"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032322"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.06.052"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.06441-11"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.026"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068708"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathophysiology30030027"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.757445"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.664377"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201016642\u20105"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129643"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.108.067645"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0201-4"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00154-5"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.25522"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22404"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.976111"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2780"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa913"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41431\u2010025\u201001802\u20103"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069861"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2011.03.002"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.866267"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147151"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22197"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.24166"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22661"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2018.00126"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.01.003"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11056"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201055506\u20107"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008640"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.514"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2014.10.011"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.3433"},{"key":"e_1_2_9_49_1","volume-title":"The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio rerio)","author":"Westerfield M.","year":"2007"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.04.035"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.16.3157"}],"container-title":["Development, Growth &amp; Differentiation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/dgd.70053","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/dgd.70053","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/dgd.70053","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T01:23:06Z","timestamp":1776993786000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/dgd.70053"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":50,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1111\/dgd.70053"],"URL":"https:\/\/doi.org\/10.1111\/dgd.70053","archive":["Portico"],"relation":{},"ISSN":["0012-1592","1440-169X"],"issn-type":[{"value":"0012-1592","type":"print"},{"value":"1440-169X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2026-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70053"}}