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In this study, we identified an RNA\u2010binding protein Ptbp1, a key component of RNA metabolism, as a critical regulator of cardiomyocyte proliferation via the formation of biomolecular condensates. We found that Ptbp1 exhibits granule\u2010like structures in cardiomyocyte nuclei during fetal to early postnatal stages. These Ptbp1 foci are phase\u2010separated liquid droplets formed via its multivalent RNA binding domains. Functional analyses revealed that Ptbp1 is essential for cardiomyocyte proliferation in neonatal mouse primary culture assays. Furthermore, proximity labeling using a primary antibody\u2010based approach combined with RIP\u2010seq in highly purified cardiomyocyte populations demonstrated that these condensates are enriched with multiple proliferation\u2010associated factors. 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