{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T01:49:23Z","timestamp":1770774563006,"version":"3.50.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T00:00:00Z","timestamp":1455235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1100123"],"award-info":[{"award-number":["1100123"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabet. Med."],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To assess serum oestrogen levels and oestrogenic activity in adolescents with Type 1 diabetes compared with a healthy control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted a cross\u2010sectional study that evaluated adolescents with Type 1 diabetes (<jats:italic>n<\/jats:italic> = 38) and healthy adolescents (control group; <jats:italic>n<\/jats:italic> = 32). Serum oestrogens, urinary oestrogen metabolites and serum oestrogenic activity were assessed. Oestrogenic activity was evaluated in an <jats:italic>in vitro<\/jats:italic> cell proliferation assay using a modified E\u2010screen assay with <jats:styled-content style=\"fixed-case\">MCF<\/jats:styled-content>\u20107\/<jats:styled-content style=\"fixed-case\">BUS<\/jats:styled-content> cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Adolescents with Type 1 diabetes had lower oestrogenic activity levels in both phases of the menstrual cycle compared with the control group (follicular phase: 76 vs 94%; luteal phase: 97 vs 131%; <jats:italic>P<\/jats:italic> &lt; 0.01), even after adjusting for <jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content>, oestradiol and oestrone levels. Postmenarcheal adolescents with Type 1 diabetes had lower oestradiol levels compared with control subjects in the follicular phase (63.3 pmol\/l vs 89.4 pmol\/l; <jats:italic>P<\/jats:italic> &lt; 0.01) and higher oestrone levels compared with controls in the luteal phase (196 vs 151.9 pmol\/l; <jats:italic>P<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Adolescents with Type 1 diabetes had lower levels of serum oestrogenic activity, and these were lower than expected based on their serum oestradiol levels. We postulate that changes in the serum milieu of oestrogens in patients with Type 1 diabetes may explain their decreased oestrogenic activity and may play a role in their adverse metabolic profile.<\/jats:p><\/jats:sec>","DOI":"10.1111\/dme.13078","type":"journal-article","created":{"date-parts":[[2016,1,23]],"date-time":"2016-01-23T14:48:35Z","timestamp":1453560515000},"page":"1366-1373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Oestrogen activity of the serum in adolescents with Type 1 diabetes"],"prefix":"10.1111","volume":"33","author":[{"given":"D.","family":"Mart\u00ednez","sequence":"first","affiliation":[{"name":"Faculty of Medicine Institute of Maternal and Child Research University of Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Castro","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Institute of Maternal and Child Research University of Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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