{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:48:26Z","timestamp":1790905706581,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T00:00:00Z","timestamp":1697760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["23\u201065\u201010014"],"award-info":[{"award-number":["23\u201065\u201010014"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabet Med"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diabetes is a chronic disorder with rapidly increasing prevalence that is a major global issue of our current era. There are two major types of diabetes. Polygenic forms of diabetes include type 1 diabetes (T1D) and type 2 diabetes (T2D) and its monogenic forms are maturity\u2010onset diabetes of the young (MODY) and neonatal diabetes mellitus (NDM). There are no permanent therapeutic approaches for diabetes and current therapies rely on regular administration of various drugs or insulin injection. Recently, gene editing strategies have offered new promise for treating genetic disorders. Targeted genome editing is a fast\u2010growing technology, recruiting programmable nucleases to specifically modify target genomic sequences. These targeted nucleases generate double\u2010strand breaks at target regions in the genome, which induce cellular repair pathways including non\u2010homologous end joining (NHEJ) and homology\u2010directed repair (HDR). Clustered regularly interspaced palindromic repeats (CRISPR)\/CRISPR\u2010associated protein 9 (Cas9) is a novel gene\u2010editing system, permitting precise genome modification. CRISPR\/Cas9 has great potential for various applications in diabetic research such as gene screening, generation of diabetic animal models and treatment. In this article, gene\u2010editing strategies are summarized with a focus on the CRISPR\/Cas9 approach in diabetes research.<\/jats:p>","DOI":"10.1111\/dme.15240","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T01:13:50Z","timestamp":1697246030000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Application of <scp>CRISPR\u2010Cas9<\/scp> technology in diabetes research"],"prefix":"10.1111","volume":"41","author":[{"given":"Malihe","family":"Lotfi","sequence":"first","affiliation":[{"name":"Medical Genetics Research Center Mashhad University of Medical Sciences  Mashhad Iran"},{"name":"Department of Medical Genetics and Molecular Medicine, Faculty of Medicine Mashhad University of Medical Sciences  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5762-3917","authenticated-orcid":false,"given":"Alexandra E.","family":"Butler","sequence":"additional","affiliation":[{"name":"Research Department Royal College of Surgeons in Ireland Bahrain  Adliya Bahrain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasily N.","family":"Sukhorukov","sequence":"additional","affiliation":[{"name":"Institute of General Pathology and Pathophysiology  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8656-1444","authenticated-orcid":false,"given":"Amirhossein","family":"Sahebkar","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center, Pharmaceutical Technology Institute Mashhad University of Medical Sciences  Mashhad Iran"},{"name":"Applied Biomedical Research Center Mashhad University of Medical Sciences  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)01301-6"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abb7107"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.6065\/apem.2019.24.3.143"},{"issue":"1","key":"e_1_2_9_5_1","first-page":"1","article-title":"An overview of the epidemiology of type 1 diabetes mellitus","volume":"2","author":"Shojaeian A","year":"2018","journal-title":"Int J Metab Syndr"},{"key":"e_1_2_9_6_1","first-page":"207","volume-title":"Genome Editing in Cardiovascular and Metabolic Diseases","author":"Shah P","year":"2022"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000002217"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-8587(18)30112-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)30058-2"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40842-016-0039-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/db09-9028"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-161192"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-018-0728-6"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.g7607"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-017-0947-3"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2021.102830"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.17352\/2455-8583.000038"},{"key":"e_1_2_9_18_1","doi-asserted-by":"crossref","unstructured":"Ga\u00e1lZ BaloghI.Monogenic forms of diabetes mellitus. 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