{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T11:27:19Z","timestamp":1774524439939,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001632","name":"Ulster University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001632","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000361","name":"Diabetes UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000361","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabet Med"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pancreatic islet \u03b2\u2010cell mass expands during pregnancy, but underlying mechanisms are not fully understood. This study examines the impact of pregnancy and cafeteria diet on islet morphology, associated cellular proliferation\/apoptosis rates as well as \u03b2\u2010cell lineage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Non\u2010pregnant and pregnant Ins<jats:italic>1<\/jats:italic><jats:sup>Cre\/+<\/jats:sup>;<jats:italic>Rosa26\u2010eYFP<\/jats:italic> transgenic mice were maintained on either normal or high\u2010fat cafeteria diet, with pancreatic tissue obtained at 18\u2009days gestation. Immunohistochemical changes in islet morphology, \u03b2\u2010\/\u03b1\u2010cell proliferation and apoptosis, as well as islet cell identity, neogenesis and ductal cell transdifferentiation were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Pregnant normal diet mice displayed an increase in body weight and glycaemia. Cafeteria feeding attenuated this weight gain while causing overt hyperglycaemia. Pregnant mice maintained on a normal diet exhibited typical expansion in islet and \u03b2\u2010cell area, owing to increased \u03b2\u2010cell proliferation and survival as well as ductal to \u03b2\u2010cell transdifferentiation and \u03b2\u2010cell neogenesis, alongside decreased \u03b2\u2010cell dedifferentiation. Such pregnancy\u2010induced islet adaptations were severely restricted by cafeteria diet. Accordingly, islets from these mice displayed high levels of \u03b2\u2010cell apoptosis and dedifferentiation, together with diminished \u03b2\u2010cell proliferation and lack of pregnancy\u2010induced \u03b2\u2010cell neogenesis and transdifferentiation, entirely opposing islet cell modifications observed in pregnant mice maintained on a normal diet.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Augmentation of \u03b2\u2010cell mass during gestation arises through various mechanisms that include proliferation and survival of existing \u03b2\u2010cells, transdifferentiation of ductal cells as well as \u03b2\u2010cell neogenesis. Remarkably, cafeteria feeding almost entirely annuls pregnancy\u2010induced islet adaptations, which may contribute to the development of gestational diabetes in the setting of dietary provoked metabolic stress.<\/jats:p><\/jats:sec>","DOI":"10.1111\/dme.15434","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T18:15:36Z","timestamp":1725992136000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cafeteria diet compromises natural adaptations of islet cell transdifferentiation and turnover in pregnancy"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1989-2403","authenticated-orcid":false,"given":"Vaibhav","family":"Dubey","sequence":"first","affiliation":[{"name":"Centre for Diabetes, School of Biomedical Sciences Ulster University  Coleraine Northern Ireland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6525-4050","authenticated-orcid":false,"given":"Neil","family":"Tanday","sequence":"additional","affiliation":[{"name":"Centre for Diabetes, School of Biomedical Sciences Ulster University  Coleraine Northern Ireland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4855-964X","authenticated-orcid":false,"given":"Nigel","family":"Irwin","sequence":"additional","affiliation":[{"name":"Centre for Diabetes, School of Biomedical Sciences Ulster University  Coleraine Northern Ireland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8883-176X","authenticated-orcid":false,"given":"Andrei I.","family":"Tarasov","sequence":"additional","affiliation":[{"name":"Centre for Diabetes, School of Biomedical Sciences Ulster University  Coleraine Northern Ireland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8548-7943","authenticated-orcid":false,"given":"Peter R.","family":"Flatt","sequence":"additional","affiliation":[{"name":"Centre for Diabetes, School of Biomedical Sciences Ulster University  Coleraine Northern Ireland UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7196-7863","authenticated-orcid":false,"given":"R. 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