{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:29:16Z","timestamp":1773016156400,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T00:00:00Z","timestamp":1762646400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20369"],"award-info":[{"award-number":["U22A20369"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["TD2022C001"],"award-info":[{"award-number":["TD2022C001"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabet Med"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aims to investigate the effects of isoastragaloside I (IAS\u2010I) on improving diabetic symptoms and its impact on endogenous \u03b2\u2010cell mass, and further explore the origins of increased \u03b2\u2010cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Healthy and diabetic mice received IAS\u2010I treatment (5 or 0.5\u2009mg\/kg, respectively) via tail veil injection. The blood glycaemic control, body weight, and glucose\/insulin tolerance were evaluated. Safety was assessed via complete blood count and histopathological analysis. Uniformly sampled pancreatic sections (spanning the entire pancreas) were immunohistochemically stained for \u03b2\u2010cells, imaged, and analysed by point\u2010counting morphometry to estimate \u03b2\u2010cell mass. Furthermore, SOX9\u2010CreER\n                      <jats:sup>T2<\/jats:sup>\n                      ; R26\u2010LSL\u2010tdTomato mice were used to trace the pancreatic ductal cell, and conduct multiplex immunohistochemistry to explore the conversion of pancreatic ductal cells into \u03b2\u2010cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In both healthy and diabetic mice, IAS\u2010I could increase the \u03b2\u2010cell mass; especially 0.5\u2009mg\/kg IAS\u2010I significantly increased the small islet mass. Using lineage tracing, we demonstrated that IAS\u2010I promoted the generation of \u03b2\u2010cells from ductal cells. We also found that IAS\u2010I could improve the fasting blood glucose and insulin resistance in diabetic mice, and the safety of IAS\u2010I in\u00a0vivo had also been preliminarily demonstrated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our results suggest that IAS\u2010I increases \u03b2\u2010cell mass and promotes the formation of \u03b2\u2010cells from pancreatic ductal cells in\u00a0vivo. It is also speculated to possess islet\u2010protective activity. These findings provide insight into the potential application of\n                      <jats:italic>Astragalus membranaceus<\/jats:italic>\n                      monomers in diabetic treatment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/dme.70162","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T04:45:49Z","timestamp":1762749949000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isoastragaloside I improves hyperglycaemia and increases \u03b2\u2010cell mass in mice"],"prefix":"10.1111","volume":"43","author":[{"given":"Ankang","family":"Pan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Utilization of Woody Oil Resource, Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and Utilization, Molecular Cell Biology Laboratory, College of Life Science Northeast Forestry University  Harbin 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Plant Bioactive Substance Biosynthesis and Utilization, Molecular Cell Biology Laboratory, College of Life Science Northeast Forestry University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhan","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Utilization of Woody Oil Resource, Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and Utilization, Molecular Cell Biology Laboratory, College of Life Science Northeast Forestry University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongsheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Utilization of Woody Oil Resource, Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and Utilization, Molecular Cell Biology Laboratory, College of Life Science Northeast Forestry University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-8393","authenticated-orcid":false,"given":"Miao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Utilization of Woody Oil Resource, Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and Utilization, Molecular Cell Biology Laboratory, College of Life Science Northeast Forestry University  Harbin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun\u2010Bo","family":"Teng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Utilization of Woody Oil Resource, Heilongjiang Key Laboratory of Plant Bioactive Substance Biosynthesis and Utilization, Molecular Cell Biology Laboratory, College of Life Science Northeast Forestry University  Harbin 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