{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T06:31:03Z","timestamp":1787639463509,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000361","name":"Diabetes UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000361","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabet Med"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>The EndoC \u03b2H5 cell line represents a promising human \u03b2\u2010cell model for preclinical diabetes research. Indeed, EndoC \u03b2H5 cells aggregate into islet\u2010like organoids, secrete insulin in a glucose\u2010dependent manner, and express a variety of \u03b2\u2010cell functional markers. However, EndoC \u03b2H5 cell survival and functionality in\u00a0vivo have not yet been investigated. Here, we have transplanted EndoC \u03b2H5 pseudoislets under the kidney capsule in SCID mice and analysed their capacity to regulate whole body blood glucose concentrations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>EndoC \u03b2H5 pseudoislets were formed in agar microwells. Oxygen consumption rate and glucose\u2010stimulated insulin secretion were quantified in\u00a0vitro. After 31\u2009days, groups of 1000 EndoC \u03b2H5 pseudoislets were transplanted under the kidney capsule in SCID mice. To selectively ablate endogenous \u03b2\u2010cells, streptozotocin was administered 1 month after transplantation, and EndoC \u03b2H5 graft function was analysed through quantification of blood glucose and human plasma insulin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>EndoC \u03b2H5 pseudoislets exhibited glucose\u2010induced insulin secretion at 15\u2009days, which was maintained at 31\u2009days of culture. Human insulin was detectable in mouse plasma at 1 week post\u2010transplantation. Despite this, streptozotocin administration led to overt diabetes in transplant recipients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>EndoC \u03b2H5 pseudoislets secrete insulin in a glucose\u2010responsive manner in\u00a0vitro, remain viable in\u00a0vivo, and secrete insulin. Despite this, transplantation of 1000 pseudoislets is not sufficient to maintain glycaemia when endogenous \u03b2\u2010cells are destroyed. Nonetheless, EndoC \u03b2H5 pseudoislets represent a useful tool for preclinical islet transplantation studies where graft control of systemic glycaemia is not a requirement.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/dme.70454","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T06:10:38Z","timestamp":1787638238000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluating\n                    <scp>EndoC \u03b2H5<\/scp>\n                    pseudoislets as a model for human islet transplantation"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0902-5664","authenticated-orcid":false,"given":"Lydia F.","family":"Daniels Gatward","sequence":"first","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0938-783X","authenticated-orcid":false,"given":"William T.","family":"Blackstone Whines","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu\u2010Wen","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8915-8774","authenticated-orcid":false,"given":"Rosie","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3645-3363","authenticated-orcid":false,"given":"Jessica","family":"Starikova","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adya","family":"Lonsane","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryea","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Salem","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1201-2335","authenticated-orcid":false,"given":"Peter M.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5759-7985","authenticated-orcid":false,"given":"Aileen F. J.","family":"King","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanta J.","family":"Persaud","sequence":"additional","affiliation":[{"name":"Department of Diabetes and Obesity, School of Cardiovascular and Metabolic Medicine and Sciences King's College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01898"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2024.115205"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/db25-0037"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.5966\/sctm.2012-0120"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2023.101772"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58447"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI129484"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI72674"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2015.09.008"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510790103"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000100401.62912.B2"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.2337\/db20-0570"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/ajc.1936.45"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0385-7_17"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181d54bce"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/dme.14974"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.6145"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2011.08.026"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00034.2016"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.115892"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-00317-7"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2024.1354199"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/19382014.2024.2406041"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2017.12.007"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2019.01.005"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/dme.15227"}],"container-title":["Diabetic Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/dme.70454","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/dme.70454","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/dme.70454","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T06:10:39Z","timestamp":1787638239000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/dme.70454"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,24]]},"references-count":26,"alternative-id":["10.1111\/dme.70454"],"URL":"https:\/\/doi.org\/10.1111\/dme.70454","archive":["Portico"],"relation":{},"ISSN":["0742-3071","1464-5491"],"issn-type":[{"value":"0742-3071","type":"print"},{"value":"1464-5491","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,24]]},"assertion":[{"value":"2026-06-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70454"}}