{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T17:23:01Z","timestamp":1783531381120,"version":"3.55.0"},"reference-count":63,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,1,13]],"date-time":"2019-01-13T00:00:00Z","timestamp":1547337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["dom-pubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the effects of long\u2010term low\u2010carbohydrate low\u2010protein ketogenic diet (KD) on cardiac metabolism and diabetic cardiomyopathy status in lean diabetic Goto\u2010Kakizaki (GK) rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Diabetic GK rats were fed with KD for 62 weeks. Cardiac function and metabolism were assessed using magnetic resonance imaging and\n                      <jats:sup>13<\/jats:sup>\n                      C magnetic resonance spectroscopy (\n                      <jats:sup>13<\/jats:sup>\n                      C\u2010MRS), at rest and under dobutamine stress.\n                      <jats:sup>13<\/jats:sup>\n                      C\u2010MRS was performed following injection of hyperpolarized [3\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]acetoacetate, [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]butyrate or [1\u2010\n                      <jats:sup>13<\/jats:sup>\n                      C]pyruvate to assess ketone body, short\u2010chain fatty acid or glucose utilization, respectively. Protein expression and cardiomyocyte structure were determined via Western blotting and histology, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>KD lowered blood glucose, triglyceride and insulin levels while increasing blood ketone body levels. In KD\u2010fed diabetic rats, myocardial ketone body and glucose oxidation were lower than in chow\u2010fed diabetic rats, while myocardial glycolysis and short\u2010chain fatty acid oxidation were unaltered. Dobutamine stress revealed an increased cardiac preload and reduced cardiac compliance in KD\u2010fed diabetic rats. Dobutamine\u2010induced stimulation of myocardial glycolysis was more enhanced in KD\u2010fed diabetic rats than in chow\u2010fed diabetic rats, which was potentially facilitated via an upregulation in basal expression of proteins involved in glucose transport and glycolysis in the hearts of KD\u2010fed rats. The metabolic profile induced by KD was accompanied by cardiac hypertrophy, a trend for increased myocardial lipid and collagen content, and an increased marker of oxidative stress.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>KD seems to exacerbate diabetic cardiomyopathy in GK rats, which may be associated with maladaptive cardiac metabolic modulation and lipotoxicity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/dom.13608","type":"journal-article","created":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T03:24:34Z","timestamp":1544498674000},"page":"949-960","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Cardiac metabolic modulation upon low\u2010carbohydrate low\u2010protein ketogenic diet in diabetic rats studied in vivo using hyperpolarized\n                    <sup>13<\/sup>\n                    C pyruvate, butyrate and acetoacetate probes"],"prefix":"10.1111","volume":"21","author":[{"given":"Desiree","family":"Abdurrachim","sequence":"first","affiliation":[{"name":"Functional Metabolism Group, Singapore Bioimaging Consortium Agency for Science, Technology, and Research  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing Qi","family":"Teo","sequence":"additional","affiliation":[{"name":"Functional Metabolism Group, Singapore Bioimaging Consortium Agency for Science, Technology, and Research  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chern Chiuh","family":"Woo","sequence":"additional","affiliation":[{"name":"Functional Metabolism Group, Singapore Bioimaging Consortium Agency for Science, Technology, and Research  Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sing Yee","family":"Ong","sequence":"additional","affiliation":[{"name":"Functional Metabolism 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