{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T16:00:52Z","timestamp":1785254452092,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T00:00:00Z","timestamp":1716940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["dom-pubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Fatty acid esters of hydroxy fatty acids (FAHFA) are a class of bioactive lipids with anti\u2010inflammatory, antidiabetic and cardioprotective properties. FAHFA hydrolysis into its fatty acid (FA) and hydroxy fatty acid (HFA) constituents can affect the bioavailability of FAHFA and its subsequent biological effects. We aimed to investigate FAHFA levels and FAHFA hydrolysis activity in children with or without obesity, and in adults with or without coronary artery disease (CAD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Our study cohort included 20 children without obesity, 40 children with obesity, 10 adults without CAD and 28 adults with CAD. We quantitated plasma levels of four families of FAHFA [palmitic acid hydroxy stearic acid (PAHSA), palmitoleic acid hydroxy stearic acid (POHSA), oleic acid hydroxy stearic acid (OAHSA), stearic acid hydroxy stearic acid] and their corresponding FA and HFA constituents using liquid chromatography\u2010tandem mass spectrometry analysis. Surrogate FAHFA hydrolysis activity was estimated as the FA\/FAHFA or HFA\/FAHFA ratio.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Children with obesity had lower plasma PAHSA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.001), OAHSA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.006) and total FAHFA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.011) levels, and higher surrogate FAHFA hydrolysis activity represented by PA\/PAHSA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.040) and HSA\/OAHSA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.025) compared with children without obesity. Adults with CAD and a history of myocardial infarction (MI) had lower POHSA levels (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.026) and higher PA\/PAHSA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.041), POA\/POHSA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.003) and HSA\/POHSA (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u2009.038) compared with those without MI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Altered FAHFA metabolism is associated with obesity and MI, and inhibition of FAHFA hydrolysis should be studied further as a possible therapeutic strategy in obesity and MI.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/dom.15686","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T03:04:56Z","timestamp":1717038296000},"page":"3429-3438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Plasma fatty acid esters of hydroxy fatty acids and surrogate fatty acid esters of hydroxy fatty acids hydrolysis activity in children with or without obesity and in adults with or without coronary artery disease"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6914-2391","authenticated-orcid":false,"given":"Sze Min","family":"Ong","sequence":"first","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine, National University of Singapore  Singapore Singapore"},{"name":"Khoo Teck Puat\u2010National University Children's Medical Institute, National University Hospital, National University Health System  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Zhi Wei","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Science, National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaddeus En Zhe","family":"Chee","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Science, National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew Anjian","family":"Sng","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine, National University of Singapore  Singapore Singapore"},{"name":"Khoo Teck Puat\u2010National University Children's Medical Institute, National University Hospital, National University Health System  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chew Kiat","family":"Heng","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine, National University of Singapore  Singapore Singapore"},{"name":"Khoo Teck Puat\u2010National University Children's Medical Institute, National University Hospital, National University Health System  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yung Seng","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine, National University of Singapore  Singapore Singapore"},{"name":"Khoo Teck Puat\u2010National University Children's Medical Institute, National University Hospital, National University Health System  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric Chun Yong","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Faculty of Science, National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delicia Shu Qin","family":"Ooi","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Yong Loo Lin School of Medicine, National University of Singapore  Singapore Singapore"},{"name":"Khoo Teck Puat\u2010National University Children's Medical Institute, National University Hospital, National University Health System  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,29]]},"reference":[{"issue":"2","key":"e_1_2_11_2_1","first-page":"142","article-title":"The epidemic of obesity and diabetes: trends and treatments","volume":"38","author":"Barnes AS","year":"2011","journal-title":"Tex Heart Inst J"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306825"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.9161"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.035"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.754387"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlr.2021.100108"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/db16-0385"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.006956"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.01.001"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.691401"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10121689"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2017.11.004"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.06.045"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2020.01.026"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2051"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.012145"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6b00565"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2020999118"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41390-022-01941-z"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2021.11.018"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000617"},{"key":"e_1_2_11_23_1","unstructured":"Health Promotion Board (HPB) S. Child Health Booklet.Health Promotion Board (HPB) Singapore.https:\/\/www.healthhub.sg\/sites\/assets\/Assets\/Programs\/parenting_portal\/pdf\/ECN_CHB_A5_72pp_Web.pdf."},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.040"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b00503"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.113.064998"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.122.001493"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/20406223211033102"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-016-0199-1"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12937-015-0102-4"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.703835"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-021-02160-5"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.783"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043732"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2013.01.004"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0237074"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1708"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.222679"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R200009-JLR200"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)32429-9"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2006-1318"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-004-1598-x"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/131.2.231"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-8223(21)08023-8"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(01)00780-8"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-43676-0_26-1"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04787-x"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b00045"}],"container-title":["Diabetes, Obesity and Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dom-pubs.pericles-prod.literatumonline.com\/doi\/pdf\/10.1111\/dom.15686","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T03:44:39Z","timestamp":1761882279000},"score":1,"resource":{"primary":{"URL":"https:\/\/dom-pubs.pericles-prod.literatumonline.com\/doi\/10.1111\/dom.15686"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,29]]},"references-count":48,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/dom.15686"],"URL":"https:\/\/doi.org\/10.1111\/dom.15686","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/DOM.15686\/v1\/review4","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/DOM.15686\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/DOM.15686\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/DOM.15686\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/DOM.15686\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/DOM.15686\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/DOM.15686\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/DOM.15686\/v2\/decision1","asserted-by":"object"}]},"ISSN":["1462-8902","1463-1326"],"issn-type":[{"value":"1462-8902","type":"print"},{"value":"1463-1326","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,29]]},"assertion":[{"value":"2024-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}