{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:42:40Z","timestamp":1772160160209,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T00:00:00Z","timestamp":1749427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["dom-pubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2025,9]]},"abstract":"Abstract<\/jats:title>\n \n Aims<\/jats:title>\n Data about long\u2010term clinical outcomes of revascularization procedures, especially for autologous cell therapy (ACT), in diabetic patients with chronic limb\u2010threatening ischaemia (CLTI) are lacking. The aim of our study was to compare the mortality and amputation rates in patients with diabetic foot ulcers (DFU) and CLTI treated by ACT with patients treated by repeated percutaneous transluminal angioplasty (re\u2010PTA) and those treated conservatively.<\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n One\u2010hundred and thirty patients with DFU and CLTI (defined as transcutaneous oxygen pressure\u2014TcPO\n 2<\/jats:sub>\n <30\u2009mmHg after unsuccessful standard revascularization) treated in our foot clinic over 9\u2009years were enrolled in the study. Forty\u2010five patients were treated by ACT, 43 patients underwent re\u2010PTA, and 42 patients were treated conservatively and formed the control group. Overall survival, amputation\u2010free survival (AFS) and major amputation rate were assessed over a 7\u2010year follow\u2010up period.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Baseline demographic characteristics and comorbidities were similar between groups. However, patients in ACT and control groups had significantly worse baseline angiograms in accordance with Graziani and GLASS (infrapopliteal region) classifications than the re\u2010PTA group (both\n p<\/jats:italic>\n \u2009<\u20090.001), but there were no differences in baseline values of TcPO\n 2<\/jats:sub>\n between groups. AFS in the ACT and re\u2010PTA groups were significantly longer compared to control (both\n p<\/jats:italic>\n \u2009<\u20090.001). The rate of major amputation was significantly lower in both active groups (both\n p<\/jats:italic>\n \u2009<\u20090.001). The re\u2010PTA group showed significantly longer overall survival compared to the control group (\n p<\/jats:italic>\n \u2009<\u20090.001), but there was no significant difference between ACT and control groups (\n p<\/jats:italic>\n \u2009=\u20090.063) and ACT and re\u2010PTA groups (\n p<\/jats:italic>\n \u2009=\u20090.081) in this parameter.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Our study showed significantly longer AFS and lower major amputation rates in patients treated by ACT and re\u2010PTA in contrast to patients treated conservatively. Overall survival was significantly longer only in the re\u2010PTA group. ACT was shown to be effective in long\u2010term limb salvage in people with no\u2010option CLTI.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/dom.16510","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T22:34:57Z","timestamp":1749508497000},"page":"4729-4736","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long\u2010term efficacy of autologous cell therapy, repeated\n PTA<\/scp>\n and conservative treatment in people with diabetes and chronic limb\u2010threatening ischaemia"],"prefix":"10.1111","volume":"27","author":[{"given":"M.","family":"Dubsk\u00fd","sequence":"first","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"},{"name":"First Faculty of Medicine Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Soj\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"},{"name":"First Faculty of Medicine Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Hus\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"},{"name":"First Faculty of Medicine Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"N\u011bmcov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"},{"name":"First Faculty of Medicine Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Fejfarov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"},{"name":"Second Faculty of Medicine Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Jaro\u0161\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"},{"name":"Second Faculty of Medicine Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Frelichov\u00e1","sequence":"additional","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"},{"name":"Second Faculty of Medicine Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Sutoris","sequence":"additional","affiliation":[{"name":"Diabetes Center Institute for Clinical and Experimental Medicine Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3186-4122","authenticated-orcid":false,"given":"E. 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