{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T20:12:50Z","timestamp":1785269570808,"version":"3.55.0"},"reference-count":69,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["dom-pubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2025,12]]},"abstract":"Abstract<\/jats:title>\n \n Aims<\/jats:title>\n \n Elevated circulating lipids are linked to cardiovascular disease (\n CVD<\/jats:styled-content>\n ), especially in insulin\u2010resistant states like polycystic ovary syndrome (\n PCOS<\/jats:styled-content>\n ), but their effects on cardiovascular risk proteins (\n CVRPs<\/jats:styled-content>\n ) remain unclear. This study used a two\u2010step approach to examine acute cardiovascular proteomic responses to lipid\u2010induced metabolic stress. We first identified proteins altered by lipids and insulin in healthy control (\n HC<\/jats:styled-content>\n ) women, then assessed whether these responses were similar or divergent in women with\n PCOS<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In a randomised cross\u2010over study, 10 healthy controls and 12 women with\n PCOS<\/jats:styled-content>\n underwent 5\u2010h saline (control) or intralipid infusions. After 3\u2009h, a 2\u2010h hyperinsulinemic\u2010euglycemic clamp was initiated. Plasma\n CVRP<\/jats:styled-content>\n expression was assessed at baseline, post\u2010lipid (180\u2009min) and post\u2010clamp (300\u2009min) using\n SOMAscan<\/jats:styled-content>\n proteomics.\n STRING<\/jats:styled-content>\n and pathway enrichment analyses were performed to explore functional associations.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In the\n HC<\/jats:styled-content>\n group, lipid infusion altered the expression of 11 out of 54\n CVRPs<\/jats:styled-content>\n including increases in\n RANK<\/jats:styled-content>\n ,\n IL2RA<\/jats:styled-content>\n ,\n TACI<\/jats:styled-content>\n ,\n SLAF5<\/jats:styled-content>\n and\n DCN<\/jats:styled-content>\n (\n p<\/jats:italic>\n <0.05) and decreases in\n THPO<\/jats:styled-content>\n ,\n BOC<\/jats:styled-content>\n ,\n SOD2<\/jats:styled-content>\n ,\n FGF23<\/jats:styled-content>\n , and\n AgRP<\/jats:styled-content>\n (\n p<\/jats:italic>\n <0.05). Most changes reversed with insulin, but\n BOC<\/jats:styled-content>\n ,\n SOD2<\/jats:styled-content>\n ,\n MMP12<\/jats:styled-content>\n ,\n FGF23<\/jats:styled-content>\n , and\n DCN<\/jats:styled-content>\n remained dysregulated. In\n PCOS<\/jats:styled-content>\n , responses mirrored the\n HC<\/jats:styled-content>\n group except for lower\n AgRP<\/jats:styled-content>\n following lipid infusion (\n p<\/jats:italic>\n <0.01), and persistent elevation of\n SLAF5<\/jats:styled-content>\n and\n DCN<\/jats:styled-content>\n following insulin (\n p<\/jats:italic>\n <0.05). Enrichment analysis linked altered proteins to immune activation, cell proliferation, and cytokine\u2010receptor signalling.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Acute lipid infusion revealed shared and phenotype\u2010specific proteomic responses linked to early vascular stress. In\n PCOS<\/jats:styled-content>\n , persistent dysregulation suggests reduced metabolic adaptability, with exploratory signals that may complement established biomarkers of early cardiovascular risk.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/dom.70154","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T09:14:38Z","timestamp":1759137278000},"page":"7464-7475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Shared and divergent acute cardiovascular risk protein responses to lipid infusion in women with and without\n PCOS<\/scp>"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0085-5338","authenticated-orcid":false,"given":"Ebrahim","family":"Rajab","sequence":"first","affiliation":[{"name":"School of Medicine Royal College of Surgeons of Ireland\u2010Medical University of Bahrain Busaiteen 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