{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:00:39Z","timestamp":1791460839134,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2017ZX09303001"],"award-info":[{"award-number":["2017ZX09303001"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370814"],"award-info":[{"award-number":["82370814"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["dom-pubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Diabetes Obesity Metabolism"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Metabolic dysfunction\u2010associated steatohepatitis (MASH) is a progressive liver disease with limited effective treatments. Glucagon\u2010like peptide\u20101 receptor agonists (GLP\u20101RAs) have shown promise for metabolic and hepatic benefits. This study evaluated the therapeutic efficacy of Efsubaglutide Alfa, a novel long\u2010acting GLP\u20101RA, in a mouse model of MASH.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Male C57BL\/6J mice with diet\u2010induced obesity received a high\u2010fat diet and low\u2010dose CCl\n                      <jats:sub>4<\/jats:sub>\n                      injections to induce MASH. Mice were randomized to receive vehicle, obeticholic acid (OCA), semaglutide or Efsubaglutide Alfa at low, medium or high doses for 42\u2009days. Endpoints included liver histology, collagen quantification by second harmonic generation\/two\u2010photon excitation fluorescence (SHG\/TPEF) imaging, and serum liver enzymes, lipids and metabolic parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      By day 41, Efsubaglutide Alfa produced dose\u2010dependent reductions in body weight, liver weight and liver\u2010to\u2010body weight ratio versus MASH controls (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). Histology showed reduced steatosis and lowered the NAFLD Activity Scores (NAS), with high\u2010dose treatment achieving a NAS of 3.0\u2009\u00b1\u20090.47 versus 4.5\u2009\u00b1\u20090.22 in MASH controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). Although Sirius Red\u2013based fibrosis area and scoring did not show significant differences among treatment groups, SHG\/TPEF imaging analysis showed lower perisinusoidal collagen metrics (%PS: 0.27%\u20130.30% vs. 0.40% in MASH controls,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Furthermore, Efsubaglutide Alfa reduced serum ALT and AST levels and improved fasting glucose and triglycerides; fasting insulin was lower in semaglutide and high\u2010dose Efsubaglutide Alfa groups, consistent with improved glycaemic control.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Efsubaglutide Alfa reduced liver steatosis and improved SHG\/TPEF\u2010derived perisinusoidal collagen features, which supported further evaluation of Efsubaglutide Alfa for MASH, particularly for steatosis improvement and suggests potential effects on fibrosis\u2010related features that warrant confirmation in longer duration studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/dom.70495","type":"journal-article","created":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T08:28:01Z","timestamp":1768897681000},"page":"3070-3081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Efsubaglutide Alfa attenuates metabolic dysfunction\u2010associated steatohepatitis in mice with improvements in second harmonic generation\u2010derived fibrosis features"],"prefix":"10.1111","volume":"28","author":[{"given":"Yahao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Huashan Hospital, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Huashan Hospital, Shanghai Medical College Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guirui","family":"Yan","sequence":"additional","affiliation":[{"name":"Innogen Pharmaceutical Co. 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