{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T15:05:48Z","timestamp":1764169548811,"version":"3.46.0"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T00:00:00Z","timestamp":1610928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Dermatologic Therapy"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Calciphylaxis is a rare condition characterized by skin ulceration and necrosis as a result of vascular calcification of the small and medium blood vessels of skin and subcutaneous tissues. It mainly occurs in patients with advanced chronic kidney disease and sometimes leads to complications with a fatal outcome. In this report, we describe the case of a 67\u2010year\u2010old male patient with end stage renal disease presenting painful skin ulcers on his lower limbs. The lesions had progressively grown and were associated to severe pain and decreased quality of life. The ulcers did not respond to conventional treatments and the patient underwent skin biopsy of these lesions obtaining anatomopathological findings compatible with calciphylaxis. In this report, we present an innovative treatment for skin ulcers secondary to calciphylaxis using cryopreserved amniotic membrane (AM) as a dressing in order to promote epithelialization of the wounds. After four applications, healing of the main ulcer and reduction in pain was achieved. In summary, applying cryopreserved AM probed to be a promising strategy to reduce pain and to enhance epithelialization and healing of chronic non\u2010responsive ulcers in calciphylaxis.<\/jats:p>","DOI":"10.1111\/dth.14769","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T15:41:05Z","timestamp":1610638865000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Use of cryopreserved human amniotic membrane in the treatment of skin ulcers secondary to calciphylaxis"],"prefix":"10.1155","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2756-8275","authenticated-orcid":false,"given":"Jos\u00e9 Antonio","family":"L\u00f3pez Mart\u00ednez","sequence":"first","affiliation":[{"name":"Department of Plastic Surgery and Burns Unit, Clinical University Hospital \u201cVirgen de la Arrixaca\u201d Biomedical Research Institute of Murcia (\u201cInstituto Murciano de Investigaci\u00f3n Biosanitaria\u201d, IMIB)  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica","family":"Rodr\u00edguez Valiente","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Complex wounds and Diabetic Foot Unit, Clinical University \u201cHospital Virgen de la Arrixaca\u201d Biomedical Research Institute of Murcia (\u201cInstituto Murciano de Investigaci\u00f3n Biosanitaria\u201d, IMIB)  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Fuente\u2010Mora","sequence":"additional","affiliation":[{"name":"Biomedical Research Institute of Murcia (\u201cInstituto Murciano de Investigaci\u00f3n Biosanitaria\u201d, IMIB) Clinical University Hospital \u201cVirgen de la Arrixaca\u201d  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Mar\u00eda","family":"Garc\u00eda\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Cell Therapy Unit, Clinical University Hospital \u201cVirgen de la Arrixaca\u201d Biomedical Research Institute of Murcia (\u201cInstituto Murciano de Investigaci\u00f3n Biosanitaria\u201d, IMIB)  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"C\u00e1novas Sanch\u00eds","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery and Burns Unit, Clinical University Hospital \u201cVirgen de la Arrixaca\u201d Biomedical Research Institute of Murcia (\u201cInstituto Murciano de Investigaci\u00f3n Biosanitaria\u201d, IMIB)  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemente Jos\u00e9","family":"Fern\u00e1ndez Pascual","sequence":"additional","affiliation":[{"name":"Department of Plastic Surgery and Burns Unit, Clinical University Hospital \u201cVirgen de la Arrixaca\u201d Biomedical Research Institute of Murcia (\u201cInstituto Murciano de Investigaci\u00f3n Biosanitaria\u201d, IMIB)  Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1505292"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.12659\/AJCR.906981"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000824"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-013-0782-z"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.13622"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfs548"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpainsymman.2006.03.009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.CR0070034"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2010.00604.x"},{"key":"e_1_2_10_11_1","first-page":"91","article-title":"The amniotic 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