{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:33:23Z","timestamp":1760783603155},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,11,26]],"date-time":"2012-11-26T00:00:00Z","timestamp":1353888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:sec><jats:label \/><jats:p>Eur J Clin Invest 2012<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Angiogenesis is a prerequisite for tumour development, progression and metastasis; however, its underlying molecular mechanisms in endometrial carcinoma are poorly understood.<\/jats:p><jats:p><jats:bold>Design\u2002<\/jats:bold> In this study, the mRNA and protein expression profiles of two key regulators of angiogenesis, vascular endothelial growth factor (VEGF) and transforming growth factor beta\u20101 (TGFB1), were evaluated by real\u2010time PCR and western blot analysis in 23 endometrial cancer tissue\u2010paired specimens (malignant vs. adjacent normal tissues). We aimed to investigate whether VEGF and TGFB1 serve as markers of the malignant transformation of the endometrium and whether VEGF or TGFB1 expression can constitute a useful prognostic marker of survival in patients with endometrial carcinoma.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Tissue\u2010pair analysis revealed VEGF transcriptional up\u2010regulation and TGFB1 mRNA down\u2010regulation as the most frequent transcriptional features. VEGF and TGFB1 mRNA were positively correlated (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001). VEGF protein levels were higher in endometrioid\u2010type tissue pairs (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0\u00b7047). TGFB1 protein and mRNA levels were negatively correlated (<jats:italic>P<\/jats:italic>\u2003=\u20030\u00b7042). TGFB1 protein expression was related to survival only in patients with endometrioid adenocarcinoma (<jats:italic>P<\/jats:italic>\u2003=\u20030\u00b7045).<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Tissue\u2010pair mRNA and protein analysis reveals VEGF transcriptional up\u2010regulation and TGFB1 down\u2010regulation that are correlated with the malignant transformation of the endometrium, while post\u2010transcriptional mechanisms control VEGF and TGFB1 protein. TGFB1 protein demonstrated a prognostic value only in endometrioid adenocarcinoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12017","type":"journal-article","created":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T10:35:17Z","timestamp":1349951717000},"page":"79-90","source":"Crossref","is-referenced-by-count":4,"title":["Prognostic value of tgfb1 protein in endometrioid adenocarcinoma"],"prefix":"10.1111","volume":"43","author":[{"given":"Giannoula","family":"Soufla","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stavros","family":"Sifakis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippos","family":"Porichis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Demetrios 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