{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T06:38:11Z","timestamp":1788503891992,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,1,7]],"date-time":"2013-01-07T00:00:00Z","timestamp":1357516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We investigated the androgen receptor (<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>) bioluminescense response in serum and urine before and after testosterone challenge in different genotypes of the <jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>2B17 enzyme, which catalyses testosterone glucuronidation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>The androgen receptor activity was determined using a yeast\u2010based bioluminescence assay. The androgens were analysed using <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>, and the individuals were genotyped for <jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>2B17 deletion polymorphism using real\u2010time <jats:styled-content style=\"fixed-case\">polymerase chain reaction<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The serum concentrations of testosterone and dihydrotestosterone (<jats:styled-content style=\"fixed-case\">DHT<\/jats:styled-content>) were markedly elevated on days 2 and 4 and were still above baseline on day 15 after a dose of 500\u00a0mg testosterone enanthate. The androgenic activity in serum increased in parallel and correlated with the hormone concentrations and remained above baseline on day 15. The urinary androgenic activity increased 4\u20135\u2010fold and was closely related to the unconjugated testosterone and independent of the <jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>2B17 genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> assay may serve as a complement to the urinary testosterone\/epitestosterone (T\/E) doping test, because this is profoundly influenced by the <jats:styled-content style=\"fixed-case\">UGT<\/jats:styled-content>2B17 deletion polymorphism. It may also be useful for detection of other illicit androgens in sports, or in the society, or for monitoring and diagnostics of androgen\u2010related disorders.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12037","type":"journal-article","created":{"date-parts":[[2013,1,2]],"date-time":"2013-01-02T06:23:14Z","timestamp":1357107794000},"page":"248-255","source":"Crossref","is-referenced-by-count":16,"title":["Testosterone challenge and androgen receptor activity in relation to <scp>UGT<\/scp>2B17 genotypes"],"prefix":"10.1111","volume":"43","author":[{"given":"Lena","family":"Ekstr\u00f6m","sequence":"first","affiliation":[{"name":"Laboratory Medicine Division Clinical Pharmacology Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Cevenini","sequence":"additional","affiliation":[{"name":"Department of Chemistry Istituto Nazionale di Biostrutture e Biosistemi University of Bologna, and INBB Roma 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