{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T05:36:49Z","timestamp":1777700209390,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,7,22]],"date-time":"2013-07-22T00:00:00Z","timestamp":1374451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Coronary artery disease (<jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content>) may reflect generalized inflammation. We evaluated leucocyte activation in subjects with and without <jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content> in different vascular compartments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Patients were divided in two groups; subjects without <jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content> (controls; <jats:italic>n\u00a0<\/jats:italic>=\u00a025) and with stable <jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content> (<jats:italic>n\u00a0<\/jats:italic>=\u00a052) based on coronary angiography. After blood sampling from vessels, cardiovascular risk factors and leucocyte activation markers <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>66b and cytoplasmatic myeloperoxidase (<jats:styled-content style=\"fixed-case\">MPO<\/jats:styled-content>) were determined by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Myeloperoxidase (<jats:styled-content style=\"fixed-case\">MPO<\/jats:styled-content>) was higher in patients with <jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content> at all sites compared with controls (188\u00a0\u00b1\u00a07 vs. 210\u00a0\u00b1\u00a012 au for venous (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00\u00b705), 178\u00a0\u00b1\u00a07 vs. 212\u00a0\u00b1\u00a012 au for femoral artery (<jats:italic>P\u00a0<\/jats:italic>=\u00a00\u00b708), 166\u00a0\u00b1\u00a07 vs. 195\u00a0\u00b1\u00a012 au for abdominal artery (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00\u00b705), 166\u00a0\u00b1\u00a06 vs. 189\u00a0\u00b1\u00a014 au for left coronary (<jats:italic>P\u00a0<\/jats:italic>=\u00a00\u00b708) and 163\u00a0\u00b1\u00a06 vs. 193\u00a0\u00b1\u00a012 au for the right coronary artery (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00\u00b705)). Other markers did not differ between the groups. A gradient of inflammation from peripheral vessels to the coronaries was found by differences in <jats:styled-content style=\"fixed-case\">MPO<\/jats:styled-content> in both groups; from 210\u00a0\u00b1\u00a012\u00a0au in the venous compartments towards 189\u00a0\u00b1\u00a014 and 193\u00a0\u00b1\u00a012\u00a0au, in the left and right coronaries, respectively, for the controls (<jats:italic>P\u00a0<\/jats:italic>=\u00a00\u00b7001), and from 188\u00a0\u00b1\u00a07 au in the venous compartment towards 166\u00a0\u00b1\u00a06 and 163\u00a0\u00b1\u00a06 au in the left and right coronaries, respectively, for the patients (<jats:italic>P\u00a0<\/jats:italic>=\u00a00\u00b7007). Other leucocyte activation markers did not show such a gradient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>There is a generalized inflammatory neutrophil gradient for <jats:styled-content style=\"fixed-case\">MPO<\/jats:styled-content> from peripheral vessels towards the coronaries in both patients with <jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content> and controls. However, patients with <jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content> show a higher degree of inflammation, mostly in the coronaries. These data strengthen the role of activated neutrophils in <jats:styled-content style=\"fixed-case\">CAD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12121","type":"journal-article","created":{"date-parts":[[2013,5,31]],"date-time":"2013-05-31T05:21:51Z","timestamp":1369977711000},"page":"920-925","source":"Crossref","is-referenced-by-count":11,"title":["Trans\u2010vessel gradient of myeloperoxidase in coronary artery disease"],"prefix":"10.1111","volume":"43","author":[{"given":"Arash","family":"Alipour","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Center for Diabetes and Vascular Medicine St. Franciscus Gasthuis Rotterdam The Netherlands"},{"name":"Department of Cardiology St. Antonius Hospital Nieuwegein The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josep","family":"Ribalta","sequence":"additional","affiliation":[{"name":"Unitat de Recerca de L\u00edpids i Arteriosclerosi Facultat de Medicina Universitat Rovira i Virgili CIBER de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM) Reus Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tjin L.","family":"Njo","sequence":"additional","affiliation":[{"name":"Department ofClinical Chemistry St Franciscus Gasthuis Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans W.","family":"Janssen","sequence":"additional","affiliation":[{"name":"Department ofClinical Chemistry St Franciscus Gasthuis Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erwin","family":"Birnie","sequence":"additional","affiliation":[{"name":"Department of Statistics and Education St Franciscus Gasthuis Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Addy J. 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