{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:04:17Z","timestamp":1772823857603,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T00:00:00Z","timestamp":1373846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Catheter\u2010related bloodstream infections (<jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>s) are currently detected with a reactive diagnostic policy, that is, application of tests to patients with clinically suspected <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>. The aim of our study was to evaluate whether <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>s could be anticipated in an earlier stage by microbiological screening using peptide nucleic acid fluorescence <jats:italic>in situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">PNA FISH<\/jats:styled-content>) with universal hybridization probes or acridine\u2010orange leucocyte cytospin (<jats:styled-content style=\"fixed-case\">AOLC<\/jats:styled-content>) tests in haemodialysis and haematological patients with <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content>s <jats:italic>in situ<\/jats:italic> compared with routine test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Peptide nucleic acid fluorescence <jats:italic>in situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">PNA FISH<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">AOLC<\/jats:styled-content> tests using blood samples from both <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> lines in patients undergoing haemodialysis were performed three times a week and from one <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> line in haematological patients were performed daily. Results were compared with those obtained from routinely performed <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> diagnostic tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One hundred fifteen patients with 139 catheter periods were investigated. The mean observation time per catheter period was 25\u00a0days (<jats:styled-content style=\"fixed-case\">IQR<\/jats:styled-content> 13\u00b75\u201343\u00b75), resulting in 5615 <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> days with a total of 4839 tested blood samples. Five <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> cases were detected by routine measures resulting in a <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> rate of 0\u00b79\/1000 catheter days. Four of five <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>s could be anticipated by positive <jats:styled-content style=\"fixed-case\">PNA FISH<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AOLC<\/jats:styled-content> tests 2\u20138\u00a0days before the diagnosis was established with routine measures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The proactive anticipative strategy using microscopic examination of <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> blood samples to anticipate <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> in an earlier stage compared with routine measures is a new diagnostic approach in patients with <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content>s and a high risk of developing <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12126","type":"journal-article","created":{"date-parts":[[2013,6,16]],"date-time":"2013-06-16T11:18:37Z","timestamp":1371381517000},"page":"964-969","source":"Crossref","is-referenced-by-count":11,"title":["Microbiological screening for earlier detection of central venous catheter\u2013related bloodstream infections"],"prefix":"10.1111","volume":"43","author":[{"given":"Jasmin","family":"Wagner","sequence":"first","affiliation":[{"name":"Section of Infectious Diseases and Tropical Medicine Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gernot","family":"Schilcher","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Zollner\u2010Schwetz","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Tropical Medicine Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Hoenigl","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Tropical Medicine Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Valentin","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Tropical Medicine Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Ribitsch","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joerg","family":"Horina","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander R.","family":"Rosenkranz","sequence":"additional","affiliation":[{"name":"Division of Nephrology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Grisold","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, Microbiology and Environmental Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Unteregger","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Troppan","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelika","family":"Valentin","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Neumeister","sequence":"additional","affiliation":[{"name":"Division of Hematology Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Krause","sequence":"additional","affiliation":[{"name":"Section of Infectious Diseases and Tropical Medicine Department of Internal Medicine Medical University of Graz Graz Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-140-1-200401060-00007"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.4065\/81.9.1159"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-132-5-200003070-00009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir257"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/1062860606294631"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.136.2.229"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1086\/657912"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70235-9"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.118-123.2003"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972-5229.92074"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa061115"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70280-9"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197706092962301"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/599376"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)11134-0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4835-4837.2004"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)04070-2"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03154.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-004-1112-x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2012.06.003"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2010.01.001"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00085-07"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.01270210"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199903000-00028"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2010.471"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0261-5614(03)00102-X"},{"key":"e_1_2_9_28_1","first-page":"34","article-title":"A prospective study of central venous hemodialysis catheter colonization and peripheral bacteremia","volume":"51","author":"Dittmer ID","year":"1999","journal-title":"Clin Nephrol"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1797.1997.tb00241.x"}],"container-title":["European Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Feci.12126","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eci.12126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T11:47:06Z","timestamp":1696333626000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eci.12126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,15]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1111\/eci.12126"],"URL":"https:\/\/doi.org\/10.1111\/eci.12126","archive":["Portico"],"relation":{},"ISSN":["0014-2972","1365-2362"],"issn-type":[{"value":"0014-2972","type":"print"},{"value":"1365-2362","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,15]]}}}