{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T19:44:11Z","timestamp":1780602251972,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T00:00:00Z","timestamp":1397692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Primary Ciliary Dyskinesia (PCD) is an orphan disease characterized by recurrent respiratory infections and an increased prevalence of situs inversus and male infertility. Low nasal Nitric Oxide (nNO) is used as a new test to diagnose PCD. The test sensitivity is good, but specificity has not been studied widely. Therefore, we evaluated the diagnostic accuracy of low nNO to diagnose PCD in a large cohort, including healthy patients and different disease controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Nasal nitric oxide was measured during plateau exhalation against resistance (nNO<jats:sub>plat<\/jats:sub>) and during tidal breathing (nNO<jats:sub>tid<\/jats:sub>). Moreover, we measured fractional exhaled NO (FENO). We included 226 patients: 38 with PCD, 49 healthy controls, and 139 disease controls (cystic fibrosis, humoral immunodeficiency, and asthma).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The nNO<jats:sub>plat<\/jats:sub> cut\u2010off value of 300\u00a0ppb provided the best sensitivity (89\u00b75%) and specificity (87\u00b73%) to detect PCD. There was overlap between PCD and disease controls: 16\u00b75% of disease controls had a false\u2010positive result. nNO<jats:sub>tid<\/jats:sub> correlated with nNO<jats:sub>plat<\/jats:sub> (<jats:italic>r<\/jats:italic>\u00a0=\u00a00\u00b7912), but values differed (<jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b70001). The nNO<jats:sub>tid<\/jats:sub> cut\u2010off of 200\u00a0ppb had a sensitivity of 89\u00b75% and a specificity of 80\u00b76% to detect PCD. The FENO cut\u2010off of 10\u00a0ppb had an acceptable sensitivity (89\u00b75%), but a low specificity (58\u00b73%). Positive and negative likelihood ratios were suboptimal for all tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>nNO<jats:sub>plat<\/jats:sub>, nNO<jats:sub>tid<\/jats:sub> and FENO measurements overlap between PCD and disease controls. Sensitivity is comparable for the three tests. Applying composite scores slightly improves diagnostic accuracy. Given the less than 90% test sensitivity, PCD should be considered in patients with intermediate results.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12254","type":"journal-article","created":{"date-parts":[[2014,3,5]],"date-time":"2014-03-05T14:53:11Z","timestamp":1394031191000},"page":"477-485","source":"Crossref","is-referenced-by-count":43,"title":["Diagnostic accuracy of nitric oxide measurements to detect primary ciliary dyskinesia"],"prefix":"10.1111","volume":"44","author":[{"given":"Mieke","family":"Boon","sequence":"first","affiliation":[{"name":"Department of Pediatrics Pediatric Pulmonology University Hospital Gasthuisberg Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Meyts","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Immunodeficiency University Hospital Gasthuisberg Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marijke","family":"Proesmans","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Pulmonology University Hospital Gasthuisberg Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francois L.","family":"Vermeulen","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Pulmonology University Hospital Gasthuisberg Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Jorissen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology, Head and Neck Surgery University Hospital Gasthuisberg Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris","family":"De Boeck","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Pediatric Pulmonology University Hospital Gasthuisberg Leuven Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00176608"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-012-1785-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00176111"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00032610"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.7.586"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00305503"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.58.1.68"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.94.07081501"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.1999.13f28.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2011.03.007"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2011.02501.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.126.4.1054"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201305-110OC"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00068810"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00044310"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2012.11.021"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.117.4.1085"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01096.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2006.096958"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/000164800750001116"},{"key":"e_1_2_8_22_1","unstructured":"Global initiative for Asthma.Pocket Guide for Asthma Management and prevention\u2010revised 2006. 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