{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:57:01Z","timestamp":1785427021103,"version":"3.56.0"},"reference-count":55,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,10,30]],"date-time":"2014-10-30T00:00:00Z","timestamp":1414627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Aintree University Hospital NHS Foundation Trust and St Helens and Knowsley NHS Foundation Trust","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Obstructive sleep apnoea (<jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content>) is a common complication of obesity and can have a substantial negative impact on a patient's quality of life and risk of cardiovascular disease. The aim of this case\u2013control study was to undertake discovery profiling of urinary peptides using capillary electrophoresis\u2013mass spectrometry (<jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) in obese subjects with and without <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content>, without a history of coronary artery disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Urinary samples were analysed by <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>. Body composition and blood pressure measurements were recorded. Overnight polysomnography was conducted to confirm or refute <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> patients were na\u00efve to continuous positive airway pressure treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sixty\u2010one subjects with <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> (age 47\u00a0\u00b1\u00a09\u00a0years, <jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content> 43\u00a0\u00b1\u00a08\u00a0kg\/m<jats:sup>2<\/jats:sup>) and 31 controls (age 49\u00a0\u00b1\u00a010\u00a0years, <jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content> 40\u00a0\u00b1\u00a05\u00a0kg\/m<jats:sup>2<\/jats:sup>) were studied; <jats:italic>P<\/jats:italic>\u00a0=\u00a0ns for age and <jats:styled-content style=\"fixed-case\">BMI<\/jats:styled-content>. Apnoea\u2013hypopnoea Index was higher in patients with <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> (24\u00a0\u00b1\u00a018\u00b76) than controls without <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> (non\u2010<jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content>) (2\u00b76\u00a0\u00b1\u00a01\u00b71; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b70001). Metabolic syndrome was present in 35 (57%) of those with <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> compared with 4 (13%) of controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b70001). Twenty\u2010four polypeptides were candidates for differential distribution (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b701), although these differences did not reach significance after multiple testing. Sequences were determined for eight peptides demonstrating origins from collagens and fibrinogen alpha.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In this study, we report for the first time, urinary proteomic profile analyses using <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> and non\u2010<jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> obese groups. The differences in urinary proteomic profiles prior to adjustment for multiple testing, with increased metabolic syndrome in obese <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content> subjects, suggest that there may be a role for <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> in characterising urinary profiles in severely obese populations with <jats:styled-content style=\"fixed-case\">OSA<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12346","type":"journal-article","created":{"date-parts":[[2014,9,30]],"date-time":"2014-09-30T08:46:50Z","timestamp":1412066810000},"page":"1104-1115","source":"Crossref","is-referenced-by-count":16,"title":["Urinary proteomics in obstructive sleep apnoea and obesity"],"prefix":"10.1111","volume":"44","author":[{"given":"Ian W.","family":"Seetho","sequence":"first","affiliation":[{"name":"Department of Obesity &amp; Endocrinology University of Liverpool Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justyna","family":"Siwy","sequence":"additional","affiliation":[{"name":"Mosaiques Diagnostics GmbH Hannover Germany"},{"name":"BHF Glasgow Cardiovascular Research Centre University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaya","family":"Albalat","sequence":"additional","affiliation":[{"name":"BHF Glasgow Cardiovascular Research Centre University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Mullen","sequence":"additional","affiliation":[{"name":"BHF Glasgow Cardiovascular Research Centre University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Mischak","sequence":"additional","affiliation":[{"name":"BHF Glasgow Cardiovascular Research Centre University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Parker","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University Hospital Aintree Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonya","family":"Craig","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University Hospital Aintree Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"Duffy","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University Hospital Aintree Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin J.","family":"Hardy","sequence":"additional","affiliation":[{"name":"Department of Diabetes &amp; Endocrinology St Helens &amp; Knowsley Teaching Hospitals St Helens UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jatin G.","family":"Burniston","sequence":"additional","affiliation":[{"name":"School of Sports &amp; Exercise Sciences Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P. 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