{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T20:55:33Z","timestamp":1761252933070},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,11,9]],"date-time":"2014-11-09T00:00:00Z","timestamp":1415491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Indian Council of Medical Research (ICMR), Government of India","award":["Project IRIS ID 2005-04430"],"award-info":[{"award-number":["Project IRIS ID 2005-04430"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Angiotensin <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (Ang <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>) and high\u2010fat diet are implicated in causing pathological changes in the vascular endothelium, brain, kidney and liver. The association of aneurysm leading to histopathological changes in the splenic compartment remains elusive. Further, the salubrious credentials of antioxidants, especially \u03b1\u2010tocopherol and \u03b2\u2010carotene in the resolution of splenic pathology have not been investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four\u2010month\u2010old <jats:italic>Apoe<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice were used in the induction of aneurysm by infusing Ang <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>, and subsequently were orally administered with \u03b1\u2010tocopherol and \u03b2\u2010carotene\u2010enriched diet for 60\u00a0days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We observed splenomegaly in Ang <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\u2010infused aneurysm and high\u2010fat diet\u2010supplemented mice as compared to normal mice. These observations were further confirmed through histopathological investigations, demonstrating splenic follicular hypertrophy. We observed a remarkable decrease in the size of spleen in \u03b1\u2010tocopherol and \u03b2\u2010carotene\u2010treated <jats:italic>Apoe<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice as compared with Ang <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\u2010treated animals. Furthermore, no marked changes in the histopathological splenic sections were seen in the \u03b2\u2010carotene\u2010treated group. However, hyperplasia and proliferation of immature lymphocytes in the follicles were observed in the \u03b1\u2010tocopherol\u2010treated animals. We found that <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+ T\u2010cell levels were increased in the high\u2010fat diet group relative to the control group and were decreased in the \u03b2\u2010carotene\u2010treated animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study provides evidence that Ang <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> infusion and high\u2010fat supplementation induces abdominal aortic aneurysm that has pathological implications to the spleen. The use of \u03b2\u2010carotene but not \u03b1\u2010tocopherol as an antioxidant markedly ameliorates the pathological changes in spleen.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12351","type":"journal-article","created":{"date-parts":[[2014,10,15]],"date-time":"2014-10-15T06:22:50Z","timestamp":1413354170000},"page":"1169-1176","source":"Crossref","is-referenced-by-count":6,"title":["High\u2010fat diet\u2010 and angiotensin <scp>II<\/scp>\u2010induced aneurysm concurrently elicits splenic hypertrophy"],"prefix":"10.1111","volume":"44","author":[{"given":"Kaliappan","family":"Gopal","sequence":"first","affiliation":[{"name":"Center for Cellular and Molecular Biology (CCMB) Hyderabad India"},{"name":"Department of Orthopaedic Surgery National Orthopaedics Center for Excellence in Research and Learning (NOCERAL) Faculty of Medicine University of Malaya Kuala Lumpur 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