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We investigated whether patients with minimal change disease (MCD<\/jats:styled-content>) and focal segmental glomerulosclerosis (FSGS<\/jats:styled-content>) have distinct circulating and urinary miRNA<\/jats:styled-content>expression profiles that could lead to potential development of noninvasive biomarkers of the disease.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>Exosome miRNA<\/jats:styled-content>s were extracted from plasma and urine samples of patients with primaryFSGS<\/jats:styled-content>(n<\/jats:italic>\u00a0=\u00a016) orMCD<\/jats:styled-content>(n<\/jats:italic>\u00a0=\u00a05) and healthy controls (n<\/jats:italic>\u00a0=\u00a05). Differences in miRNA<\/jats:styled-content>abundance were examined using Affymetrix GeneChip miRNA<\/jats:styled-content>3.0 arrays.QRT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>was used to validate the findings from the array.<\/jats:p><\/jats:sec>Results<\/jats:title>Comparison analysis ofFSGS<\/jats:styled-content>versusMCD<\/jats:styled-content>revealed 126 and 155 differentially expressed miRNA<\/jats:styled-content>s in plasma and in urine, respectively. Only 38 of these miRNA<\/jats:styled-content>s were previously cited, whereas the remaining miRNA<\/jats:styled-content>s have not been described. Comparison analysis showed that a significant number of miRNA<\/jats:styled-content>s were downregulated in both plasma and urine samples of patients withFSGS<\/jats:styled-content>compared to those withMCD<\/jats:styled-content>. Plasma levels of miR\u201030b, miR\u201030c, miR\u201034b, miR\u201034c and miR\u2010342 and urine levels of mir\u20101225\u20105p were upregulated in patients withMCD<\/jats:styled-content>compared to patients withFSGS<\/jats:styled-content>and controls (P<\/jats:italic>\u00a0<\u00a00\u00b7001). Urinary levels of mir\u20101915 and miR\u2010663 were downregulated in patients withFSGS<\/jats:styled-content>compared toMCD<\/jats:styled-content>and controls (P<\/jats:italic>\u00a0<\u00a00\u00b7001), whereas the urinary levels of miR\u2010155 were upregulated in patients withFSGS<\/jats:styled-content>when compared to patients withMCD<\/jats:styled-content>and controls (P<\/jats:italic>\u00a0<\u00a00\u00b7005).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Patients withFSGS<\/jats:styled-content>andMCD<\/jats:styled-content>have a unique circulating and urinary miRNA<\/jats:styled-content>profile. The diagnostic and prognostic potential of miRNA<\/jats:styled-content>s inFSGS<\/jats:styled-content>andMCD<\/jats:styled-content>warrants further studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12420","type":"journal-article","created":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T11:00:34Z","timestamp":1423825234000},"page":"394-404","source":"Crossref","is-referenced-by-count":101,"title":["Circulating and urinary microRNA<\/scp>profile in focal segmental glomerulosclerosis: a pilot study"],"prefix":"10.1111","volume":"45","author":[{"given":"Ali","family":"Ramezani","sequence":"first","affiliation":[{"name":"Division of Renal Diseases and Hypertension The George Washington University School of Medicine Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph M.","family":"Devaney","sequence":"additional","affiliation":[{"name":"Division of Renal Diseases and Hypertension The George Washington University School of Medicine Washington DC 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