{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T02:05:56Z","timestamp":1781316356940,"version":"3.54.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T00:00:00Z","timestamp":1429833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Invasive aspergillosis is an infection with high morbidity and mortality that affects mostly immunocompromised individuals. Early identification and targeted treatment of the infection is essential to improve survival of affected patients. The purpose of our review is to highlight the most recent developments in diagnosis and screening for invasive aspergillosis (IA) along with the challenges associated with the development and validation of novel diagnostic approaches.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ovid <jats:styled-content style=\"fixed-case\">MEDLINE<\/jats:styled-content> and The Cochrane library were searched for studies that evaluated serologic, molecular and novel methodologies for the diagnosis of IA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Traditional diagnostic approaches, such as histopathology and culture, are still considered the gold standard but lack sufficient sensitivity. Newer serologic techniques, such as galactomannan (GM) and beta\u2010glucan, have already been incorporated into clinical guidelines, but recent evidence suggests that their performance might be limited in certain clinical settings. Molecular methods, such as the <jats:italic>Aspergillus<\/jats:italic> spp. polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>), have not yet found their place in clinical practice mainly due to lack of standardization. Novel methodologies, such as volatile organic compound detection and lateral flow devices, have recently been developed and promise noninvasive and rapid diagnosis of aspergillosis, while diagnostic algorithms that incorporate both GM and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> have proven to be effective in early randomized trials as screening methods and can reduce the use of antifungal agents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Diagnosis of IA remains challenging. Novel methodologies and the standardization of GM and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> might provide more reliable diagnostic tools in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12448","type":"journal-article","created":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T05:09:07Z","timestamp":1428469747000},"page":"646-652","source":"Crossref","is-referenced-by-count":50,"title":["Diagnosis of invasive aspergillosis: recent developments and ongoing challenges"],"prefix":"10.1111","volume":"45","author":[{"given":"Marios","family":"Arvanitis","sequence":"first","affiliation":[{"name":"Infectious Diseases Division Rhode Island Hospital Providence RI USA"},{"name":"Warren Alpert Medical School of Brown University Providence RI USA"},{"name":"Internal Medicine Department Boston Medical Center Boston MA 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