{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T19:18:21Z","timestamp":1774725501247,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T00:00:00Z","timestamp":1435536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Austrian Research Promotion Agency (FFG)","award":["FFG bridge project 832079"],"award-info":[{"award-number":["FFG bridge project 832079"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Catheter\u2010related bloodstream infections (<jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>s) are currently detected in patients with clinically suspicion. The aim of our study was to evaluate whether <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>s could be anticipated and detected in a subclinical stage by peptide nucleic acid fluorescence in\u00a0situ hybridization (<jats:styled-content style=\"fixed-case\">PNA FISH<\/jats:styled-content>) using universal hybridization probes or acridine orange leucocyte cytospin (<jats:styled-content style=\"fixed-case\">AOLC<\/jats:styled-content>) tests in haematooncological patients with central venous catheters (<jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content>s) in\u00a0situ.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Peptide nucleic acid fluorescence in\u00a0situ hybridization and <jats:styled-content style=\"fixed-case\">AOLC<\/jats:styled-content> tests using blood samples from one <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> lumen\/port chamber in haematooncological patients were continuously performed. These results were compared to those obtained from routinely performed <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> diagnostic tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One hundred and eighty\u2010two patients with 342 catheter periods were investigated. Seventeen <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> cases were detected in 6466 <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> days by routine measures resulting in a <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> rate of 2\u00b76\/1000 catheter days. Two of 17 showed positive <jats:styled-content style=\"fixed-case\">PNA FISH<\/jats:styled-content> tests, and five positive <jats:styled-content style=\"fixed-case\">AOLC<\/jats:styled-content> test results before the diagnosis were established with routine measures. The screening revealed further seven patients with positive universal <jats:styled-content style=\"fixed-case\">PNA FISH<\/jats:styled-content> tests and 10 positive <jats:styled-content style=\"fixed-case\">AOLC<\/jats:styled-content> tests without symptoms indicative for infection and were therefore considered not to have <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Sampling of only one <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> lumen\/port chamber screening for <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content> in haematooncological patients seems not to be a useful tool for anticipative diagnosis of <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>. Reasons for false\u2010negative results might include origin of <jats:styled-content style=\"fixed-case\">CRBSI<\/jats:styled-content>s from the other <jats:styled-content style=\"fixed-case\">CVC<\/jats:styled-content> lumina not sampled for screening, and false\u2010positive results might origin from catheter colonization without subsequent spread of micro\u2010organisms into the peripheral bloodstream.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12477","type":"journal-article","created":{"date-parts":[[2015,6,9]],"date-time":"2015-06-09T16:57:14Z","timestamp":1433869034000},"page":"824-832","source":"Crossref","is-referenced-by-count":9,"title":["Detection of central venous catheter\u2010related bloodstream infections in haematooncological patients"],"prefix":"10.1111","volume":"45","author":[{"given":"Jasmin","family":"Rabensteiner","sequence":"first","affiliation":[{"name":"Section\u00a0of Infectious Diseases and Tropical Medicine Department of Internal Medicine Medical University 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