{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:33:06Z","timestamp":1791289986795,"version":"4.3.0"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,1,21]],"date-time":"2016-01-21T00:00:00Z","timestamp":1453334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004963","name":"Seventh Framework Programme","doi-asserted-by":"publisher","award":["HEALTH\u2010F2\u20102009\u2010241544"],"award-info":[{"award-number":["HEALTH\u2010F2\u20102009\u2010241544"]}],"id":[{"id":"10.13039\/501100004963","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2016,3]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>MicroRNA<\/jats:styled-content>s (miRNA<\/jats:styled-content>s) contribute to chronic kidney disease (CKD) progression via regulating mRNA<\/jats:styled-content>s involved in renal homeostasis. However, their association with clinical outcome remains poorly understood.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>We performed miRNA<\/jats:styled-content> and mRNA<\/jats:styled-content> expression profiling on renal biopsy sections by qPCR<\/jats:styled-content> (miRNA<\/jats:styled-content>) and microarrays (mRNA<\/jats:styled-content>) in a discovery (n<\/jats:italic>\u00a0=\u00a043) and in a validation (n<\/jats:italic>\u00a0=\u00a029) cohort. miRNA<\/jats:styled-content>s differentiating stable and progressive cases were inversely correlated with putative target mRNA<\/jats:styled-content>s, which were further characterized by pathway analysis using KEGG<\/jats:styled-content> pathways.<\/jats:p><\/jats:sec>Results<\/jats:title>miR\u201030d, miR\u2010140\u20103p, miR\u2010532\u20103p, miR\u2010194, miR\u2010190, miR\u2010204 and miR\u2010206 were downregulated in progressive cases. These seven miRNA<\/jats:styled-content>s correlated with upregulated 29 target mRNA<\/jats:styled-content>s involved in inflammatory response<\/jats:italic>, cell\u2013cell interaction<\/jats:italic>, apoptosis<\/jats:italic> and intra\u2010cellular signalling<\/jats:italic>. In particular, miR\u2010206 and miR\u2010532\u20103p were associated with distinct biological processes via the expression of their target mRNA<\/jats:styled-content>s: Reduced expression of miR\u2010206 in progressive disease correlated with the upregulation of target mRNA<\/jats:styled-content>s participating in inflammatory pathways (CCL<\/jats:styled-content>19, CXCL<\/jats:styled-content>1, IFNAR<\/jats:styled-content>2, NCK<\/jats:styled-content>2, PTK<\/jats:styled-content>2B, PTPRC<\/jats:styled-content>, RASGRP<\/jats:styled-content>1 and TNFRSF<\/jats:styled-content>25). Progressive cases also showed a lower expression of miR\u2010532\u20103p and an increased expression of target transcripts involved in apoptosis pathways (MAP<\/jats:styled-content>3K14, TNFRSF<\/jats:styled-content>10B\/TRAIL<\/jats:styled-content>\u2010R2, TRADD<\/jats:styled-content> and TRAF<\/jats:styled-content>2). In the validation cohort, we confirmed the decreased expression of miR\u2010206 and miR\u2010532\u20103p, and the inverse correlation of these miRNA<\/jats:styled-content>s with the expression of nine of the 12 target genes. The levels of the identified miRNA<\/jats:styled-content>s and the target mRNA<\/jats:styled-content>s correlated with clinical parameters and histological damage indices.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These results suggest the involvement of specific miRNA<\/jats:styled-content>s and mRNA<\/jats:styled-content>s in biological pathways associated with the progression of CKD.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12585","type":"journal-article","created":{"date-parts":[[2015,12,28]],"date-time":"2015-12-28T06:24:44Z","timestamp":1451283884000},"page":"213-226","source":"Crossref","is-referenced-by-count":104,"title":["Renal microRNA<\/scp>\u2010 and RNA<\/scp>\u2010profiles in progressive chronic kidney disease"],"prefix":"10.1111","volume":"46","author":[{"given":"Michael","family":"Rudnicki","sequence":"first","affiliation":[{"name":"Department of Internal Medicine IV \u2013 Nephrology and Hypertension Medical University Innsbruck Innsbruck 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