{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T10:38:10Z","timestamp":1761993490113,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T00:00:00Z","timestamp":1481068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Impaired microvascular function leads to a poor outcome in a variety of medical conditions. Our aim was to determine whether vasodilator responses to acetylcholine (Ach) are impaired in human omental arterioles from patients with severe trauma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Patients with massive blood loss and severe shock requiring damage control procedures were included. Tissues were collected at the first (<jats:styled-content style=\"fixed-case\">FEL<\/jats:styled-content>) and the second explorative laparotomy (<jats:styled-content style=\"fixed-case\">SEL<\/jats:styled-content>). Control tissues were collected from nontrauma patients. Freshly isolated 50\u2013200\u2010\u03bcm\u2010diameter omental arterioles were analysed using videomicroscopy. Dihydroethidine and <jats:styled-content style=\"fixed-case\">DCF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content> fluorescence were used to assess reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) production. Mn<jats:styled-content style=\"fixed-case\">TBAP<\/jats:styled-content> was used to determine the contribution of excess vascular superoxide contribution to endothelial dysfunction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After constriction (30\u201350%) with endothelin\u20101, dilation to graded doses of Ach (10<jats:sup>\u22129<\/jats:sup>\u201310<jats:sup>\u22124<\/jats:sup> M) was greater in control vessels compared to <jats:styled-content style=\"fixed-case\">FEL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SEL<\/jats:styled-content> (max dilation at 10<jats:sup>\u22124<\/jats:sup> M (<jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content>) = 25 \u00b1 3%, <jats:italic>n<\/jats:italic> = 8; and 59 \u00b1 8%, <jats:italic>n<\/jats:italic> = 8, respectively, and controls <jats:styled-content style=\"fixed-case\">MD<\/jats:styled-content> = 93 \u00b1 10%, <jats:italic>n<\/jats:italic> = 6, <jats:italic>P<\/jats:italic> &lt; 0\u00b705). Fluorescence imaging of <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> production showed significant increases in superoxide (225\u00b746 \u00b1 12\u00b786; 215\u00b777 \u00b1 10\u00b775 vs. 133\u00b775 \u00b1 7\u00b726, arbitrary units; <jats:italic>P<\/jats:italic> &lt; 0\u00b705) and peroxide\u2010related <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> (240\u00b78 \u00b1 20\u00b742; 234\u00b759 \u00b1 28\u00b786, vs. 150\u00b778 \u00b1 15\u00b765, arbitrary units; <jats:italic>P<\/jats:italic> &lt; 0\u00b705), in <jats:styled-content style=\"fixed-case\">FEL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SEL<\/jats:styled-content> microvessels compared to control, respectively. <jats:styled-content style=\"fixed-case\">FEL<\/jats:styled-content> pretreated with Mn<jats:styled-content style=\"fixed-case\">TBAP<\/jats:styled-content> demonstrated significant improvement in Ach\u2010induced vasodilation (25\u00b75 \u00b1 3\u00b70% vs. 79\u00b75 \u00b1 8\u00b72%; <jats:italic>P<\/jats:italic> &lt; 0\u00b705).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Severe shock associated with microvascular endothelial dysfunction enhances production of <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> in human omental tissues. The altered flow regulation may contribute to a mismatch between local blood supply and demand, exacerbating abnormal tissue perfusion and function.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12697","type":"journal-article","created":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T13:03:06Z","timestamp":1481115786000},"page":"30-37","source":"Crossref","is-referenced-by-count":5,"title":["Shock associated with endothelial dysfunction in omental microvessels"],"prefix":"10.1111","volume":"47","author":[{"given":"Lewis B.","family":"Somberg","sequence":"first","affiliation":[{"name":"Division of Trauma\/Critical Care Departments of Medicine and Surgery Medical College of Wisconsin Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David D.","family":"Gutterman","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine Departments of Medicine and Surgery Medical College of Wisconsin Milwaukee WI 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