{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T08:31:46Z","timestamp":1723278706885},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,13]],"date-time":"2017-03-13T00:00:00Z","timestamp":1489363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University of Cassino and SL","award":["FAR 2015"],"award-info":[{"award-number":["FAR 2015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The oxidation of methionine residue in position 35 of Ab to sulphoxide (Ab\u2010sulphoxide) has the ability to deeply modify wild\u2010type Ab 1\u201342 (Ab) neurotoxic action. Our previous studies suggest that in nucleated cells, lower toxicity of Ab\u2010sulphoxide might result not from structural alteration, but from elevation of methionine sulphoxide reductase A (MsrA) activity and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>On this basis, we hypothesised that red blood cell (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>), a cell devoid almost completely of MsrA activity, shares with nucleated cells an antioxidant system induced by methionine 35 sulphoxide, responsible for the lower toxicity of Ab\u2010sulphoxide in <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>. (Results) Supporting this hypothesis, we found that the low toxicity of Ab\u2010sulphoxide in <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> correlated with pentose phosphate pathway (<jats:styled-content style=\"fixed-case\">PPP<\/jats:styled-content>) flux increase, and this event was associated with a low level of methionine oxidation in total proteins. None of these effects were observed when cells were exposed to Ab native.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>These results outline the importance of the redox state of methionine 35 in the modulation of Ab\u2010mediated events and suggest an important protective role for <jats:styled-content style=\"fixed-case\">PPP<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content> of patients affected by Alzheimer's disease.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12735","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T15:32:44Z","timestamp":1486567964000},"page":"314-321","source":"Crossref","is-referenced-by-count":3,"title":["Methionine 35 sulphoxide reduces toxicity of A\u03b2 in red blood cell"],"prefix":"10.1111","volume":"47","author":[{"given":"Cristiana","family":"Carelli\u2010Alinovi","sequence":"first","affiliation":[{"name":"School of Medicine Biochemistry and 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