{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:20:32Z","timestamp":1792412432304,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,6,14]],"date-time":"2017-06-14T00:00:00Z","timestamp":1497398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>High density lipoprotein (<jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content>) cholesterol is not decreased in hypercortisolism despite high triglycerides, which may be ascribed to effects on the cholesteryl ester transfer protein (<jats:styled-content style=\"fixed-case\">CETP<\/jats:styled-content>) pathway. We explored if <jats:styled-content style=\"fixed-case\">CETP mRNA<\/jats:styled-content> expression is modulated by glucocorticoid treatment <jats:italic>in vitro<\/jats:italic>. Effects of doubling the hydrocortisone (<jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content>) replacement dose on plasma <jats:styled-content style=\"fixed-case\">CETP<\/jats:styled-content> activity, and <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> characteristics were tested in patients with secondary adrenal insufficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Human <jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\u20101 macrophages were incubated with corticosterone <jats:italic>in vitro<\/jats:italic> in the presence or absence of a liver X receptor (<jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content>) agonist, followed by determination of <jats:styled-content style=\"fixed-case\">CETP mRNA<\/jats:styled-content> levels by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. In addition, a randomised double\u2010blind cross\u2010over study was performed in 47 patients with secondary adrenal insufficiency (university medical setting; 10 weeks exposure to a higher <jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content> dose (0\u00b74\u20130\u00b76 mg\/kg body weight) vs. 10 weeks of a lower <jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content> dose (0\u00b72\u20130\u00b73 mg\/kg body weight).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Corticosterone dose dependently decreased <jats:styled-content style=\"fixed-case\">CETP mRNA<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\u20101 macrophages. Corticosterone also decreased <jats:styled-content style=\"fixed-case\">CETP mRNA<\/jats:styled-content> expression after <jats:styled-content style=\"fixed-case\">LXR<\/jats:styled-content> pretreatment. In patients, <jats:styled-content style=\"fixed-case\">CETP<\/jats:styled-content> activity decreased with doubling of the <jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content> dose (<jats:italic>P<\/jats:italic> = 0\u00b7049), coinciding with an increase in <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> cholesterol, apolipoprotein A\u2010I and the <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> cholesterol\/apolipoprotein A\u2010I ratio (reflecting <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> size; <jats:italic>P<\/jats:italic> &lt; 0\u00b701 for each). The increase in the <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> cholesterol\/apolipoprotein A\u2010I ratio was correlated with the decrease in plasma <jats:styled-content style=\"fixed-case\">CETP<\/jats:styled-content> activity (<jats:italic>r<\/jats:italic> = \u22120\u00b7442, <jats:italic>P<\/jats:italic> = 0\u00b7002).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Glucocorticoids downregulate <jats:styled-content style=\"fixed-case\">CETP<\/jats:styled-content> gene expression in a human macrophage cell system. In line, a higher glucocorticoid replacement dose decreases plasma <jats:styled-content style=\"fixed-case\">CETP<\/jats:styled-content> activity in patients, thereby contributing to higher <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> cholesterol and an increase in estimated <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content> size.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12770","type":"journal-article","created":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T16:58:16Z","timestamp":1495731496000},"page":"494-503","source":"Crossref","is-referenced-by-count":22,"title":["Downregulation of cholesteryl ester transfer protein by glucocorticoids: a randomised study on <scp>HDL<\/scp>"],"prefix":"10.1111","volume":"47","author":[{"given":"Jorien","family":"Werumeus Buning","sequence":"first","affiliation":[{"name":"Department of Endocrinology University Medical Center Groningen University of Groningen Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidya G.","family":"Dimova","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Center for Liver, Digestive, and Metabolic Diseases Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank G.","family":"Perton","sequence":"additional","affiliation":[{"name":"Department of Endocrinology University Medical Center Groningen University of Groningen Groningen the Netherlands"},{"name":"Laboratory Center University Medical Center Groningen University of Groningen Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe J. 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