{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T01:34:01Z","timestamp":1784770441004,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,7,18]],"date-time":"2017-07-18T00:00:00Z","timestamp":1500336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Homing Plus","award":["2013-8\/4"],"award-info":[{"award-number":["2013-8\/4"]}]},{"name":"Foundation for Polish Science and EU Structural Funds","award":["2015\/16\/S\/NZ6\/00041"],"award-info":[{"award-number":["2015\/16\/S\/NZ6\/00041"]}]},{"name":"National Science Centre, Poland"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2017,8]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Systemic sclerosis (SS<\/jats:styled-content>c) is a chronic autoimmune disease characterised by tissue fibrosis and immune abnormalities. Recent evidence suggests that activated circulating monocytes from patients withSS<\/jats:styled-content>c play an important role in early stages ofSS<\/jats:styled-content>c pathogenesis due to enhanced expression of tissue inhibitor of metalloproteinases 1 (TIMP<\/jats:styled-content>\u20101),IL<\/jats:styled-content>\u20108 and reactive oxygen species (ROS<\/jats:styled-content>) induction. However, the exact factors that contribute to chronic inflammation and subsequently fibrosis progression are still unknown.<\/jats:p><\/jats:sec>Materials and methods<\/jats:title>The expression pattern ofIL<\/jats:styled-content>\u20108,TIMP<\/jats:styled-content>\u20101,AP<\/jats:styled-content>\u20101 transcription factor\u2010Fra2 andROS<\/jats:styled-content>induction in peripheral blood monocytes followingDZN<\/jats:styled-content>ep (histone methyltransferase inhibitor) andTLR<\/jats:styled-content>8 agonist stimulation was investigated. Exogenous microRNA<\/jats:styled-content>\u20105196, which is predicted to bind 3\u2032UTR<\/jats:styled-content>of Fra2 gene, was delivered to reverse profibrotic phenotype in monocytes. Expression of circulating microRNA<\/jats:styled-content>\u20105196 was correlated withSS<\/jats:styled-content>c parameters.<\/jats:p><\/jats:sec>Results<\/jats:title>DZN<\/jats:styled-content>ep\u00a0+\u00a0TLR<\/jats:styled-content>8 agonist stimulation enhanced profibroticTIMP<\/jats:styled-content>\u20101,IL<\/jats:styled-content>\u20108 andROS<\/jats:styled-content>generation inHC<\/jats:styled-content>andSS<\/jats:styled-content>c monocytes. As opposed by the decrease of miRNA<\/jats:styled-content>\u20105196 and antioxidantSOD<\/jats:styled-content>1 expression inSS<\/jats:styled-content>c monocytes. Exogenous delivery of microRNA<\/jats:styled-content>\u20105196 reduced Fra2 andTIMP<\/jats:styled-content>\u20101 expression suggesting that it may be used as a potential modulator of fibrogenesis inSS<\/jats:styled-content>c. Circulating microRNA<\/jats:styled-content>\u20105196 was significantly increased inSS<\/jats:styled-content>c and positively correlated withCRP<\/jats:styled-content>level but not with Rodnan skin score orESR<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These results suggest that microRNA<\/jats:styled-content>\u20105196 can be used as a potential biomarker characterisingSS<\/jats:styled-content>c. Overall, this study may open new possibilities for the development of microRNA<\/jats:styled-content>\u20105196\u2010based diagnostics and therapy in early phases ofSS<\/jats:styled-content>c.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12776","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:48:40Z","timestamp":1498092520000},"page":"555-564","source":"Crossref","is-referenced-by-count":29,"title":["The role of microRNA<\/scp>\u20105196 in the pathogenesis of systemic sclerosis"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0961-6130","authenticated-orcid":false,"given":"Marzena","family":"Ciechomska","sequence":"first","affiliation":[{"name":"Department of Immunology and Pathophysiology National Institute of Geriatrics Rheumatology and Rehabilitation Warsaw Poland"},{"name":"Laboratory of Clinical Immunogenetics and Pharmacogenetics Hirszfeld Institute of Immunology and Experimental Therapy of the Polish 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