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This regulation occurs by inside\u2010out and outside\u2010in signalling via interaction of cytoplasmic proteins with the intracellular domains of the integrin \u03b1\u2010 and \u03b2\u2010subunits. The underlying molecular mechanisms regulating \u03b2<jats:sub>2<\/jats:sub> integrins in neutrophils are still incompletely understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>This review provides a comprehensive overview of our current knowledge on proteins interacting with the cytoplasmic tail of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>18, the conserved \u03b2\u2010subunit of \u03b2<jats:sub>2<\/jats:sub> integrins, their regulation and their functional importance for neutrophil trafficking during acute inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 22 proteins including Talin, Kindlin 3 and Coronin 1A have been reported to interact with the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>18 cytoplasmic tail. Here, proteins binding to the cytoplasmic domain of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>18 in experiments using purified, recombinant proteins or peptides in, for example, pull\u2010down assays, are defined as direct interactors. Proteins that have been shown to interact with the cytoplasmic domain of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>18 using whole cell lysates in, for example, pull\u2010down experiments are claimed as interacting proteins without evidence for direct interaction. In summary, \u03b2<jats:sub>2<\/jats:sub> integrin activation and signalling depend on a specific subset of proteins interacting with <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>18 and their precise regulation. If disturbed, profound defects of neutrophil recruitment and activation become evident compromising the innate immune response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The knowledge of proteins interacting with \u03b2<jats:sub>2<\/jats:sub> integrins and their regulation during neutrophil trafficking does not only improve our basic understanding of innate immunity but may pave the way to novel therapeutic strategies in the treatment of inflammatory diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.12966","type":"journal-article","created":{"date-parts":[[2018,6,13]],"date-time":"2018-06-13T08:25:06Z","timestamp":1528878306000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Intracellular \u03b2<sub>2<\/sub> integrin (<scp>CD<\/scp>11\/<scp>CD<\/scp>18) interacting partners in neutrophil 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