{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:46:54Z","timestamp":1773391614203,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81550047"],"award-info":[{"award-number":["81550047"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760748"],"award-info":[{"award-number":["81760748"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatic fibrosis is a worldwide incurable disease; due to the complex and unclear mechanism, there lack the effective therapeutic targets. However, the mechanism of miR\u201023a\u20105p underling this pathological process is largely not clear. The purpose of this study was to investigate the role of miR\u201023a\u20105p in hepatic fibrosis and HSC activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The content of miR\u201023a\u20105p in hepatic fibrosis induced by N\u2010nitrosodimethylamine (NDMA) and HSC activation induced by platelet\u2010derived growth factor (PDGF) was detected by qRT\u2010PCR. H&amp;E staining, Masson staining and Shear wave electrography (SWE) were used to detect the degree of hepatic fibrosis. Immunohistochemistry staining, qRT\u2010PCR and Western blot detect the related markers of liver fibrosis or HSC activation, as well as the related pathway genes and proteins. Dual\u2010luciferase reporter system verifies the interaction between miR\u201023a\u20105p with PTEN or miR\u201023a\u20105p with lncRNA LOC102551149 in HSC\u2010T6. siRNA and miRNA mimic transfer to HSC\u2010T6 to detect the function of lncRNA LOC102551149 and miR\u201023a\u20105p on HSC activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After hepatic fibrosis and HSC activation happened, the expression of miR\u201023a\u20105p was up\u2010regulated, whereas anti\u2010miR\u201023a\u20105p can alleviate hepatic fibrosis and HSC activation. Further research shows miR\u201023a\u20105p can target PTEN and degrade it, causing activation of PI3K\/Akt\/mTOR\/Snail pathway. lncRNA LOC102551149 can be used as a competition endogenous RNA (ceRNA) targeting miR\u201023a\u20105p through base pairing, and siRNA LOC102551149 or exogenous miR\u201023a\u20105p can induce HSC activation through PI3K\/Akt\/mTOR\/Snail pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We demonstrate mechanism pathway of miR\u201023a\u20105p on hepatic fibrosis and HSC activation, which may develop a therapeutic target for hepatic fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.13243","type":"journal-article","created":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T06:25:30Z","timestamp":1587277530000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Identification lncRNA LOC102551149\/miR\u201023a\u20105p pathway in hepatic 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