{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T17:53:39Z","timestamp":1778176419761,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T00:00:00Z","timestamp":1619481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Circular RNAs (circRNAs) function as vital regulators in multifarious cancers, including hepatocellular carcinoma (HCC). However, the roles of circRNA Wolf\u2010Hirschhorn syndrome candidate gene\u20101 (circWHSC1) in HCC are barely known.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was conducted for the levels of circWHSC1, miR\u2010142\u20103p, miR\u2010421, miR\u2010665 and homeobox A1 (HOXA1) mRNA. Cell Counting Kit\u20108 (CCK\u20108) assay, colony formation assay and 5\u2032\u2010ethynyl\u20102\u2032\u2010deoxyuridine (EdU) assay were used to evaluate cell proliferation ability. Transwell assay was adopted for cell migration and invasion. Western blot assay was employed for protein levels. RNA pull\u2010down assay, dual\u2010luciferase reporter assay and RNA immunoprecipitation (RIP) assay were executed to verify the interaction between miR\u2010142\u20103p and circWHSC1 or HOXA1. Murine xenograft model assay was conducted for the role of circWHSC1 in vivo. The morphology of exosomes was observed by transmission electron microscopy (TEM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CircWHSC1 was elevated in HCC tissues and cells, and high level of circWHSC1 was associated with worse overall survival of HCC patients. Knockdown of circWHSC1 suppressed HCC cell proliferation and metastasis in vitro and restrained tumorigenesis in vivo. CircWHSC1 functioned as the sponge for miR\u2010142\u20103p, which directly targeted HOXA1. Inhibition of miR\u2010142\u20103p ameliorated the effects of circWHSC1 knockdown on HCC cell proliferation and metastasis. Moreover, miR\u2010142\u20103p overexpression restrained the growth and motility of HCC cells, with HOXA1 elevation reversing the impacts. Additionally, circWHSC1 was increased in HCC patients\u2019 serum and might be a diagnostic indicator for HCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CircWHSC1 played a tumour\u2010promoting role in HCC by elevating HOXA1 through sponging miR\u2010142\u20103p.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.13487","type":"journal-article","created":{"date-parts":[[2021,1,10]],"date-time":"2021-01-10T12:17:09Z","timestamp":1610281029000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["CircWHSC1 serves as an oncogene to promote hepatocellular carcinoma progression"],"prefix":"10.1111","volume":"51","author":[{"given":"Pengfei","family":"Lyu","sequence":"first","affiliation":[{"name":"Department of General Surgery Shanxi Provincial Cancer Hospital Taiyuan City Shanxi Province 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