{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T08:56:47Z","timestamp":1783587407260,"version":"3.55.0"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Distinct faecal microbiota profiles are reported to be associated with various subtypes of IBS. Circulating antibodies to cytolethal distending toxin B (CdtB) and vinculin are proposed as biomarkers to identify post\u2010infectious IBS. The aim of our study was to analyse serum levels of anti\u2010CdtB and anti\u2010vinculin antibodies in patients with different functional gastrointestinal disorders (FGID) and their correlation with the composition of faecal microbiome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study cohort comprised 65 prospectively recruited individuals: 15 with diarrhoea\u2010type\u2010IBS (IBS\u2010D), 13 with constipation\u2010type\u2010IBS (IBS\u2010C), 15 with functional dyspepsia (FD) and 22\u00a0healthy controls. FGID subgroups were defined according to Rome III criteria. Serum levels of anti\u2010CdtB and anti\u2010vinculin antibodies were measured by ELISA. Faecal microbiome composition analysis and assessment of dysbiosis were performed by GA\u2010map\u00ae Dysbiosis Test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Positivity rate either for anti\u2010CdtB or anti\u2010vinculin antibodies was higher in the IBS\u2010C group (76.9%) compared to IBS\u2010D (40.0%), FD (60%) and healthy (63.6%) groups.<\/jats:p><jats:p>Dysbiosis was more frequent in subjects positive for anti\u2010CdtB antibodies and in IBS\u2010C patients, who showed an increased amount of opportunistic\/pro\u2010inflammatory bacteria and reduced gut protective bacteria. IBS\u2010C patients showed a high inter\u2010individual variation of bacterial communities compared to other FGID subgroups and healthy individuals, whereas microbial profiles of patients with IBS\u2010D and FD were overlapping with those of healthy controls. No bacteria markers showed significant differences between FGID subgroups and healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Neither anti\u2010CdtB\/anti\u2010vinculin antibodies nor faecal microbial profiles allowed to discriminate between specific FGID subgroups. Dysbiosis was more frequent in patients presenting with anti\u2010CdtB antibodies and in IBS\u2010C patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.13666","type":"journal-article","created":{"date-parts":[[2021,8,14]],"date-time":"2021-08-14T13:07:05Z","timestamp":1628946425000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Gut microbiota profiles and the role of anti\u2010CdtB and anti\u2010vinculin antibodies in patients with functional gastrointestinal disorders (FGID)"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7150-5864","authenticated-orcid":false,"given":"Riccardo","family":"Vasapolli","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Infectious Diseases Otto\u2010von\u2010Guericke\u2010University Magdeburg Magdeburg Germany"},{"name":"Department of Internal Medicine II Hospital of the Ludwig Maximilians University of 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