{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:21:02Z","timestamp":1785853262003,"version":"3.56.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,7,10]],"date-time":"2022-07-10T00:00:00Z","timestamp":1657411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ageing is accompanied by a decrease in endogenous hydrogen sulphide (H<jats:sub>2<\/jats:sub>S) synthesis and the development of mitochondrial dysfunction. The aim of our work was to study the possible participation of exercise training\u2010induced regulation of endogenous H<jats:sub>2<\/jats:sub>S production in the restoration of mitochondrial function in old rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Male rats were divided into three groups: adult, old and exercise\u2010trained old. Exercise training of old rats was performed for 4\u2009weeks. The mRNA expression cystathionine\u2010\u03b3\u2010lyase (CSE) and 3\u2010mercaptopyruvate sulfurtransferase (3\u2010MST) were determined using reverse transcription and real\u2010time polymerase chain reaction analysis. Mitochondrial dysfunction was determined by mPTP opening, which was investigated by spectrophotometric registration of the swelling of mitochondria isolated from the rat heart. We also studied the effect of exercise on H<jats:sub>2<\/jats:sub>S content, oxidative stress and mtNOS activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Exercise training in old animals significantly increased the expression of H<jats:sub>2<\/jats:sub>S\u2010synthesizing enzymes CSE and 3\u2010MST and restored endogenous H<jats:sub>2<\/jats:sub>S production in cardiac tissue and cardiac mitochondria to levels of adult animals. In addition, the training significantly reduced oxidative stress in old rats, in particular the rate of formation of \u2022O<jats:sub>2<\/jats:sub><jats:sup>\u2212<\/jats:sup> and H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, diene conjugates and malondialdehyde levels in the mitochondria of the heart. Simultaneously, in the hearts of these animals, resistance of mPTP to the inducer of its opening of calcium ions was increased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Thus, exercise training restores endogenous H<jats:sub>2<\/jats:sub>S production, and significantly reduces oxidative stress in cardiac mitochondria of old rats that are associated with the inhibition of calcium\u2010induced mPTP opening as an indicator of mitochondrial dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.13829","type":"journal-article","created":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T06:33:25Z","timestamp":1656743605000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Exercise restores endogenous <scp>H<sub>2<\/sub>S<\/scp> synthesis and mitochondrial function in the heart of old rats"],"prefix":"10.1111","volume":"52","author":[{"given":"Natali\u0456a","family":"Strutynska","sequence":"first","affiliation":[{"name":"Department of Blood Circulation, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruslan","family":"Strutynskyi","sequence":"additional","affiliation":[{"name":"Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3943-4768","authenticated-orcid":false,"given":"Lidiia","family":"Mys","sequence":"additional","affiliation":[{"name":"Department of Blood Circulation, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Luchkova","sequence":"additional","affiliation":[{"name":"Department of Blood Circulation, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuliia","family":"Korkach","sequence":"additional","affiliation":[{"name":"Department of Blood Circulation, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia","family":"Goshovska","sequence":"additional","affiliation":[{"name":"Department of Blood Circulation, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Snizhana","family":"Chorna","sequence":"additional","affiliation":[{"name":"Department of Physiology and Cell Biology Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadym","family":"Sagach","sequence":"additional","affiliation":[{"name":"Department of Blood Circulation, Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2015.07.009"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00917.2020"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.15407\/fz62.02.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.316306"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.15407\/fz57.06.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.15407\/fz59.06.003"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26216463"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.15407\/fz59.04.016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.15407\/fz59.03.003"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/eci.13683"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11060896"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202002675R"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00017.2011"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/758358"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2009.02234.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2019-0411"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2030551"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1984.tb08158.x"},{"key":"e_1_2_9_20_1","first-page":"60","article-title":"Measurement of diene conjugates in blood plasma using the UV absorption of heptane and isopropanol extracts","volume":"2","author":"Gavrilov VB","year":"1988","journal-title":"Lab Delo"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(78)90342-1"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22042194"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2018-0153"},{"issue":"2","key":"e_1_2_9_24_1","first-page":"49","article-title":"Aging\u2010related increase of sensitivity of the mitochondrial permeability transition pore to inductors in the rat heart","volume":"50","author":"Sahach VF","year":"2004","journal-title":"Fiziol Zh"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-006-9192-9"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3715037"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.11.014"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.09.006"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/hh2201.100205"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.15407\/fz58.01.013"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox7100129"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/10641963.2018.1445752"}],"container-title":["European Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eci.13829","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/eci.13829","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eci.13829","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T07:18:25Z","timestamp":1692688705000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eci.13829"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,10]]},"references-count":31,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1111\/eci.13829"],"URL":"https:\/\/doi.org\/10.1111\/eci.13829","archive":["Portico"],"relation":{},"ISSN":["0014-2972","1365-2362"],"issn-type":[{"value":"0014-2972","type":"print"},{"value":"1365-2362","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,10]]},"assertion":[{"value":"2022-05-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13829"}}