{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:33:16Z","timestamp":1773660796850,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T00:00:00Z","timestamp":1668556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 2015\u2010492"],"award-info":[{"award-number":["PIP 2015\u2010492"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dysregulated hyaluronic acid (HA) metabolism has been shown to be implicated in several pathologies including endometriosis. 4\u2010Methylumbelliferone (4MU) is an HA synthesis inhibitor with proven antitumour activity. In this study, we aim to evaluate the effect of 4MU on endometriosis development both in vivo and in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Endometriosis was surgically induced by uterine tissue auto\u2010transplantation in 32 two\u2010month\u2010old BALB\/c mice. Animals were designated into the early or late starting treatment group, which initiated on day 2 or day 15 after surgery, respectively. Within each group, 4MU 200\u2009mg\/kg\/day or vehicle (Control) were administered by oesophageal gavage for 28\u2009days. After sacrifice, the percentage of developed lesions, lesion size, cell proliferation, vascularization and HA deposition within the endometriotic\u2010like lesions were evaluated. Cell viability was assessed in endometrial epithelial cells (ECC\u20101) and in endometrial stromal cells (t\u2010HESC); and migration was evaluated in t\u2010HESC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was a significant reduction in the percentage of developed lesions in mice that started the 4MU treatment on day 2 compared with its respective control group, and compared with those that started treatment on day 15. However, no significant changes were found when analysing endometriotic\u2010like lesion's cell proliferation, vascularization and HA deposition. In vitro, both cell viability and migration were inhibited by 4MU treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The inhibition of HA synthesis could be a beneficial and alternative option to treat endometriosis at the early stage of the disease. Further research is necessary to elucidate 4MU's mechanism of action and better strategies for delivering this promising drug.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.13899","type":"journal-article","created":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T16:11:36Z","timestamp":1667923896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of pharmacological inhibition of hyaluronic acid synthesis on experimental endometriosis"],"prefix":"10.1111","volume":"53","author":[{"given":"Carla Noem\u00ed","family":"Olivares","sequence":"first","affiliation":[{"name":"Laboratorio de Fisiopatolog\u00eda Endometrial Instituto de Biolog\u00eda y Medicina Experimental (IBYME \u2010 CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anal\u00eda Gabriela","family":"Ricci","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunolog\u00eda de la 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