{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:57:47Z","timestamp":1791874667454,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hydroxytyrosol reduces low\u2010density lipoprotein oxidation, contributing to prevention of atherosclerosis progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In a prospective, crossover, double\u2010blind, placebo\u2010controlled trial, 30 chronic coronary artery syndrome (CCAS) patients were randomized to 4 capsules\/day, containing 412.5\u00a0mg olive oil with 2.5\u00a0mg hydroxytyrosol (OOHT) each one or placebo for 1\u2009month and then were crossed over to the alternate treatment (placebo or OOHT). We measured (a) perfused boundary region (PBR) of the sublingual arterial microvessels (increased PBR indicates reduced glycocalyx thickness), (b) flow\u2010mediated dilation (FMD), (c) Coronary Flow Reserve (CFR) and markers of LV diastolic function by Doppler echocardiography, (d) pulse wave velocity (PWV), and (e) oxidative stress, inflammatory biomarkers and blood lipids at baseline and after treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment with OOHT improved PBR, FMD, CFR and PWV compared to baseline (1.8\u00a0\u00b1\u2009.3 vs. 1.7\u00a0\u00b1\u00a0.4\u00a0\u03bcm, <jats:italic>p<\/jats:italic>\u00a0=\u2009.040, 3.7\u00a0\u00b1\u20092.1 vs. 6.5%\u2009\u00b1\u20092.3%, <jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.001, 2.3\u00a0\u00b1\u2009.4 vs. 2.5\u00a0\u00b1\u00a0.4, <jats:italic>p<\/jats:italic>\u00a0=\u2009.030 and 11.1\u00a0\u00b1\u20091.8 vs. 11.8\u00a0\u00b1\u20092.3\u00a0m\/s, <jats:italic>p<\/jats:italic>\u00a0=\u2009.002) while there was no effect after placebo (<jats:italic>p<\/jats:italic>\u00a0=\u2009NS). No effect of OOHT treatment was observed on blood pressure. There was a parallel improvement of E' of the mitral annulus and deceleration time of the E wave of mitral inflow after OOHT (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05) but not after placebo. Compared to baseline, treatment with OOHT reduced malondialdehyde, a marker of lipid peroxidation, oxidized LDL, triglycerides, PCSK9 and CRP blood levels (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.05) in contrast to placebo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hydroxytyrosol\u2010enriched olive oil may have beneficial effects on endothelial, arterial and LV diastolic function likely by reducing oxidative and inflammatory burden in CCAS, though further studies are needed to confirm this mechanism.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.13983","type":"journal-article","created":{"date-parts":[[2023,3,13]],"date-time":"2023-03-13T10:27:24Z","timestamp":1678703244000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Association of hydroxytyrosol enriched olive oil with vascular function in chronic coronary disease"],"prefix":"10.1111","volume":"53","author":[{"given":"Ignatios","family":"Ikonomidis","sequence":"first","affiliation":[{"name":"Laboratory of Echocardiography and Preventive Cardiology, Second Cardiology Department Attikon University Hospital, National and Kapodistrian University of Athens, Medical School  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1631-5210","authenticated-orcid":false,"given":"Konstantinos","family":"Katogiannis","sequence":"additional","affiliation":[{"name":"Laboratory of Echocardiography and Preventive Cardiology, Second Cardiology Department Attikon University Hospital, National and Kapodistrian University of Athens, Medical School  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Chania","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Faculty of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos","family":"Iakovis","sequence":"additional","affiliation":[{"name":"Department of Cardiology University Hospital of Larissa  Larissa Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Tsoumani","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Faculty of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andriana","family":"Christodoulou","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Faculty of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelia","family":"Brinia","sequence":"additional","affiliation":[{"name":"Laboratory of Pharmacology, Faculty of Pharmacy National and Kapodistrian University of Athens  Athens 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