{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:32:33Z","timestamp":1753878753136,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The vasculature function is mainly regulated by the autonomic nervous system. Importantly, the sensory\u2010motor nervous system also innervates peripheral vessels and has the capacity to modulate vascular tone. Here we investigated the effects of electrical stimulation of a mixed nerve trunk on blood flow in deep arteries and muscle perfusion. Our hypothesis is that stimulation of a mixed nerve can modify blood flow.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010nine healthy participants were included into a randomized\u2010crossover and blinded clinical trial. Each subject received a placebo and two percutaneous peripheral nerve stimulation (pPNS) protocols on the median nerve: Pain Threshold continuous Low Frequency (PT\u2010cLF) and Sensory Threshold burst High Frequency (ST\u2010bHF). Blood flow was then assessed bilaterally using Power Doppler Ultrasonography at the main arteries of the arm, and blood perfusion at the forearm muscles. Afterwards, blood flow was quantified using a semi\u2010automatized software, freely shared here.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Placebo, consisting in needle insertion, produced an immediate and generalized reduction on peak systolic velocity in all arteries. Although nerve stimulation produced mainly no effects, some significant differences were found: both protocols increased the relative perfusion area of the forearm muscles, the ST\u2010bHF protocol prevented the reduction in peak systolic velocity and TAMEAN of the radial artery produced by the control protocol and PT\u2010cLF produced a TAMEAN reduction of the ulnar artery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Therefore, the arterial blood flow in the arm is mainly impervious to the electrical stimulation of the median nerve, composed by autonomic and sensory\u2010motor axons, although it produces mild modifications in the forearm muscles perfusion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/eci.14091","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T09:26:38Z","timestamp":1694078798000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Blood flow effects of percutaneous peripheral nerve stimulation. A blinded, randomized clinical trial"],"prefix":"10.1111","volume":"54","author":[{"given":"Nuria","family":"Viudes\u2010Sarri\u00f3n","sequence":"first","affiliation":[{"name":"Skeletal Biology and Engineering Research Center KU Leuven  Leuven Belgium"},{"name":"Human Movement Biomechanics Research Group, Deptartment of Movement Sciences KU Leuven  Leuven Belgium"},{"name":"Neuroscience in Physiotherapy (NiP) Independent Research Group  Elche Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Aleixandre\u2010Carrera","sequence":"additional","affiliation":[{"name":"Neuroscience in Physiotherapy (NiP) Independent Research Group  Elche Spain"},{"name":"Instituto de Neurociencias de Alicante Universidad Miguel Hern\u00e1ndez\u2010CSIC  San Juan de Alicante Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Beltr\u00e1","sequence":"additional","affiliation":[{"name":"Neuroscience in Physiotherapy (NiP) Independent Research Group  Elche Spain"},{"name":"Physical Therapy Department Valencia University  Valencia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Javier","family":"Ortega","sequence":"additional","affiliation":[{"name":"Physical therapy and advanced rehabilitation clinic RehAv Elche  Elche Spain"},{"name":"Physical Therapy Department, Health Sciences Faculty CEU\u2010Cardenal Herrera University CEU Universities  Elche Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Javier","family":"Molina\u2010Pay\u00e1","sequence":"additional","affiliation":[{"name":"Physical Therapy Department, Health Sciences Faculty CEU\u2010Cardenal Herrera University CEU Universities  Elche Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7299-0750","authenticated-orcid":false,"given":"Enrique","family":"Velasco","sequence":"additional","affiliation":[{"name":"Neuroscience in Physiotherapy (NiP) Independent Research Group  Elche Spain"},{"name":"Laboratory of Ion Channel Research, Department of Cellular and Molecular Medicine KU Leuven; 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