{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:48:31Z","timestamp":1787014111664,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T00:00:00Z","timestamp":1711756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100018543","name":"Natural Science Foundation of Tibet Autonomous Region","doi-asserted-by":"publisher","award":["XZ202101ZR0060G"],"award-info":[{"award-number":["XZ202101ZR0060G"]}],"id":[{"id":"10.13039\/501100018543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>High\u2010altitude pulmonary oedema (HAPE) is a form of noncardiogenic pulmonary oedema. Studies have found that long noncoding RNA (lncRNA) plays an important role in HAPE. ANRIL is significant in pulmonary illnesses, which implies that alterations in ANRIL expression levels may be involved in the beginning and development of HAPE. However, the specific mechanism is indistinct. The present study is meant to explore the effect and mechanism of ANRIL on hypoxic\u2010induced injury of pulmonary microvascular endothelial cells (PMEVCs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In the hypoxic model of PMVECs, overexpression of ANRIL or knockdown of miR\u2010181c\u20105p was performed to assess cell proliferation, apoptosis, and migration. Furthermore, the levels of apoptosis\u2010related proteins, inflammatory factors, and vascular active factors were also measured.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The results showed that, after 24\u2009h of hypoxia, PMVECs proliferation and migration were suppressed in comparison to the control group, along with an increase in apoptosis, a decrease in the expression of ANRIL, and an increase in the expression of miR\u2010181c\u20105p (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). The damage caused by hypoxia in PMVECs can be lessened by overexpressing ANRIL, which also inhibits the production of TNF\u2010\u03b1, iNOS, and VEGF as well as BAX and cleaved caspase\u20103 (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). Further experimental results showed that overexpression of ANRIL and knockdown of miR\u2010181c\u20105p had the same protection against hypoxic injury in PMVECs (all\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study suggests that ANRIL may prevent hypoxia injury to PMVECs in HAPE through the negative regulation of miR\u2010181c\u20105p.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/eci.14202","type":"journal-article","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T04:49:10Z","timestamp":1711774150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Long noncoding\n                    <scp>RNA ANRIL<\/scp>\n                    alleviates hypoxia\u2010induced pulmonary microvascular endothelial cell damage"],"prefix":"10.1111","volume":"54","author":[{"given":"Yijin","family":"Qi","sequence":"first","affiliation":[{"name":"School of Medicine Xizang Minzu University  Xianyang Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine Xizang Minzu University  Xianyang Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine Xizang Minzu University  Xianyang Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Medicine Xizang Minzu University  Xianyang Shaanxi 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