{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:34:10Z","timestamp":1787589250633,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pulsed field ablation (PFA) is an effective alternative to radiofrequency ablation (RFA) for pulmonary vein isolation (PVI) in atrial fibrillation (AF), offering procedural advantages and a favourable safety profile. However, single\u2010shot PFA (ssPFA) has been associated with biochemical evidence of haemolysis and myocardial injury, raising concerns regarding potential renal impairment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this prospective observational study, 40 patients undergoing first\u2010time PVI were analysed (20 RFA and 20 pentaspline ssPFA). Blood samples were obtained pre\u2010procedure and 1, 4 and 24\u2009h post\u2010ablation. Markers of myolysis (myoglobin), haemolysis (haptoglobin and bilirubin), renal function (creatinine, eGFR and Cystatin C) and tubular stress (NGAL and KIM\u20101) were assessed. Biomarker trajectories were analysed using two\u2010way repeated\u2010measures ANOVA and correlation analyses were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Significantly higher postprocedural myoglobin levels, greater reductions in haptoglobin and greater elevations in bilirubin were observed after ssPFA than after RFA (all interaction effects\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Changes in renal function and tubular stress biomarkers were modest and largely comparable between modalities. Exploratory correlation analyses demonstrated inverse associations between changes in myoglobin and eGFR, with the strongest correlation observed at 24\u2009h (\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u2009=\u2009\u22120.55). The association between early myoglobin release and subsequent eGFR changes was confined to the ssPFA cohort.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ssPFA induces substantially greater acute myolysis and haemolysis than RFA following PVI. No patient met serum creatinine\u2010defined criteria of acute kidney injury within the observation period. Myoglobin release was associated with transient renal functional changes and may represent a mechanistically plausible, non\u2010causal marker of early renal stress following ssPFA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/eci.70248","type":"journal-article","created":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T08:21:37Z","timestamp":1786177297000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Early Hemolysis, Myolysis and Renal Stress Following Pentaspline Pulsed Field Versus Radiofrequency Ablation of Atrial Fibrillation"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8420-7319","authenticated-orcid":false,"given":"Paul Felix","family":"Harbich","sequence":"first","affiliation":[{"name":"3rd Medical Department for Cardiology and Intensive Care Medicine Clinic Ottakring  Vienna Austria"},{"name":"Cluster for Cardiovascular Research Ludwig Boltzmann 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