{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:34:02Z","timestamp":1787589242016,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Evaluative measures, helping to decide whether a marginal graft can be used for transplantation or rather be discarded are of utmost importance but yet basically underdeveloped. Recent technical developments allow for the isolated detection of 13C\u2010labelled carbon dioxide in the oxygenator exhaust of an isolated perfused organ. By adding a 13C\u2010labelled tracer, its metabolism can be monitored by measuring the increase in 13C carbon in the exhaust air. Here we evaluated the potential of the technique to evaluate pig livers during machine perfusion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Porcine livers (six per group) were subjected to 5, 20 or 45\u2009min of warm ischemia and cold stored overnight. Evaluative machine perfusion was done with MPS at 20\u00b0C for 2\u2009h. After injection of 13C\u2010methacetin as a tracer,\n                      <jats:sup>13<\/jats:sup>\n                      CO\n                      <jats:sub>2<\/jats:sub>\n                      as metabolic end\u2010product can be quantified by high precision laser\u2010based spectroscopy in the gas outflow of the oxygenator.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The\n                      <jats:sup>13<\/jats:sup>\n                      CO\n                      <jats:sub>2<\/jats:sub>\n                      test showed clear differences between all three groups, and provided superior discriminative potential when compared to conventional parameters (e.g., flow, transaminases, lactate). ROC curve analysis for discrimination between 5 and 20\u2009min of warm ischemia revealed an area under the curve of 0.916 while comparison of 20 and 45\u2009min of warm ischemia results in an area under the curve of 1.0 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Thus, the 13C turnover test appears to have the potential of an objective, quantitative and valuable adjunct in the toolbox for pre\u2010transplant evaluation of questionable liver grafts.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/eci.70250","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T02:54:23Z","timestamp":1786676063000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Liver Grafts During Machine Perfusion at 20\u00b0C via Metabolization of\n                    <scp>13C<\/scp>\n                    \u2010Methacetin"],"prefix":"10.1111","volume":"56","author":[{"given":"Charlotte","family":"von Horn","sequence":"first","affiliation":[{"name":"Surgical Research Department University Hospital Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Malkus","sequence":"additional","affiliation":[{"name":"Surgical Research Department University Hospital Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derya","family":"Berger","sequence":"additional","affiliation":[{"name":"Surgical Research Department University Hospital Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefanie","family":"Bertram","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Hospital Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7124-3590","authenticated-orcid":false,"given":"Thomas","family":"Minor","sequence":"additional","affiliation":[{"name":"Surgical Research Department University Hospital Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.12659\/AOT.938132"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000003857"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0047-9"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-02641-0"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000003763"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00631"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14687"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110642"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.104311"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181ad85b5"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1477-2574.2009.00151.x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12235"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092059"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43856-023-00422-6"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.26574"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199306000-00011"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16251-3"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.04.031"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14110"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.04.019"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0000000000005156"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8091421"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12727"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo9110246"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43856-026-01736-x"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2011.09.003"},{"issue":"7","key":"e_1_2_12_28_1","first-page":"509","article-title":"Organ Donation by Maastricht\u2010III Pediatric Patients: Recommendations of the Groupe Francophone de R\u00e9animation et Urgences P\u00e9diatriques (GFRUP) and Association des Anesth\u00e9sistes R\u00e9animateurs P\u00e9diatriques d'Expression Fran\u00e7aise (ADARPEF). Part II: Specific Organizational and Technical Considerations","volume":"29","author":"Dauger S.","year":"2022","journal-title":"Archives de P\u00e9diatrie"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000153508.71684.99"},{"issue":"2","key":"e_1_2_12_30_1","first-page":"361","article-title":"Fibrinolysis in Organ Procurement for Transplantation After Cardiocirculatory Compromise","volume":"90","author":"Minor T.","year":"2003","journal-title":"Thrombosis and Haemostasis"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199805150-00019"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181a63c10"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/ctr.13101"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.25291"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.26092"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40472-018-0178-9"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425255.2.6.875"}],"container-title":["European Journal of Clinical Investigation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eci.70250","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/eci.70250","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/eci.70250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:59:58Z","timestamp":1787587198000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/eci.70250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":36,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/eci.70250"],"URL":"https:\/\/doi.org\/10.1111\/eci.70250","archive":["Portico"],"relation":{},"ISSN":["0014-2972","1365-2362"],"issn-type":[{"value":"0014-2972","type":"print"},{"value":"1365-2362","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70250"}}