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The Chinese softshell turtle <jats:italic>Pelodiscus sinensis<\/jats:italic> (Reptilia: Testudines: Trionychidae) has a distinctive limb morphology: the anterior side of the limbs has partially webbed fingers with claw\u2010like protrusions, while the posterior fingers are completely enclosed in webbings. Here, <jats:italic>P. sinensis<\/jats:italic> embryos were investigated to gain insights on the evolution of limb\u2010shaping mechanisms in amniotes. We found cell death and cell senescence in their interdigital webbings. Spatial or temporal modulation of these processes were correlated with the appearance of indentations in the webbings, but not a complete regression of this tissue. No differences in interdigital cell proliferation were found. In subsequent stages, differential growth of the finger cartilages led to a major difference in limb shape. While no asymmetry in bone morphogenetic protein signaling was evident during interdigital cell death stages, some components of this pathway were expressed exclusively in the clawed digit tips, which also had earlier ossification. In addition, a delay and\/or truncation in the chondrogenesis of the posterior digits was found in comparison with the anterior digits of <jats:italic>P. sinensis<\/jats:italic>, and also when compared with the previously published pattern of digit skeletogenesis of turtles without posterior webbings. In conclusion, modulation of cell death, as well as a heterochrony in digit chondrogenesis, may contribute to the formation of the unique limbs of the Chinese softshell turtles.<\/jats:p>","DOI":"10.1111\/ede.12352","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T07:51:16Z","timestamp":1600069876000},"page":"451-462","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Regulation of the limb shape during the development of the Chinese softshell turtles"],"prefix":"10.1111","volume":"22","author":[{"given":"Ingrid R.","family":"Cordeiro","sequence":"first","affiliation":[{"name":"Department of Life Science and Technology Tokyo Institute of Technology  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reiko","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Life Science and Technology Tokyo Institute of Technology  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8092-8594","authenticated-orcid":false,"given":"Mikiko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Life Science and Technology Tokyo Institute of Technology  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,9]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.1371\/journal.pgen.0020216"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.1126\/science.1168755"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1002\/dvdy.22619"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1016\/0012-1606(77)90176-2"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.1371\/journal.pone.0052781"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_1","DOI":"10.1111\/j.1440-169X.2007.00951.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_1","DOI":"10.1016\/S0012-1606(03)00066-6"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_1","DOI":"10.1038\/nature13496"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_1","DOI":"10.1016\/j.devcel.2019.05.025"},{"doi-asserted-by":"publisher","key":"e_1_2_8_11_1","DOI":"10.1002\/jmor.20226"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_1","DOI":"10.1038\/nprot.2009.191"},{"doi-asserted-by":"publisher","key":"e_1_2_8_13_1","DOI":"10.1007\/s13127-010-0019-x"},{"key":"e_1_2_8_14_1","first-page":"285","article-title":"Interdigital cell death during limb development of the turtle and lizard with an interpretation of evolutionary significance","volume":"40","author":"Fallon J. 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L.","year":"2012","journal-title":"Journal of Visualized Experiments"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_1","DOI":"10.1006\/dbio.1997.8843"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_1","DOI":"10.1242\/dev.00710"},{"doi-asserted-by":"publisher","key":"e_1_2_8_19_1","DOI":"10.1242\/dev.041954"},{"doi-asserted-by":"publisher","key":"e_1_2_8_20_1","DOI":"10.1002\/jez.b.22579"},{"key":"e_1_2_8_21_1","first-page":"15","article-title":"The onset of osteogenesis in the developing chick limb","volume":"44","author":"Holder N.","year":"1978","journal-title":"Journal of Embryology and Experimental Morphology"},{"doi-asserted-by":"publisher","key":"e_1_2_8_22_1","DOI":"10.1038\/ncomms12903"},{"doi-asserted-by":"publisher","key":"e_1_2_8_23_1","DOI":"10.1016\/j.ydbio.2015.12.016"},{"doi-asserted-by":"publisher","key":"e_1_2_8_24_1","DOI":"10.1086\/377110"},{"doi-asserted-by":"publisher","key":"e_1_2_8_25_1","DOI":"10.1111\/j.1525-142X.2005.05002.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_26_1","DOI":"10.18632\/aging.100844"},{"doi-asserted-by":"publisher","key":"e_1_2_8_27_1","DOI":"10.1242\/dev.02172"},{"doi-asserted-by":"publisher","key":"e_1_2_8_28_1","DOI":"10.1007\/BF00187925"},{"doi-asserted-by":"publisher","key":"e_1_2_8_29_1","DOI":"10.1242\/dev.126.23.5515"},{"issue":"11","key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1242\/dev.128.11.2075","article-title":"Role of FGFs in the control of programmed cell death during limb development","volume":"128","author":"Montero J. 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