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Recent advances in single\u2010cell transcriptomics (scRNA\u2010seq) have significantly improved our understanding of NCC biology. However, their dynamic migratory behavior and spatiotemporal heterogeneity in the developing embryo pose significant challenges for their identification and isolation. Consequently, most studies of NCCs have been confined to model organisms with established transgenic tools or established methods for\n                    <jats:italic>in ovo<\/jats:italic>\n                    manipulation. To overcome this limitation, we present a novel approach that combines antibody labeling with fluorescence activated cell sorting to enrich for NCCs and we demonstrate the approach in the common wall lizard (\n                    <jats:italic>Podarcis muralis<\/jats:italic>\n                    ). Through microscopy, reverse transcription quantitative polymerase chain reaction and single\u2010cell RNA sequencing, we show that the method enriches for NCCs as efficiently as methods relying on transgenic animals. Using this technique, we successfully characterize transcriptional profiles of NCCs in wall lizard embryos. We anticipate that this method can be applied to a wide range of vertebrates that lack transgenic tools, enabling deeper insights into the diverse roles of neural crest cells in development and evolution.\n                  <\/jats:p>","DOI":"10.1111\/ede.70030","type":"journal-article","created":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T05:40:42Z","timestamp":1771479642000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enrichment of Neural Crest Cells by Antibody Labeling and Flow Cytometry for Single\u2010Cell Transcriptomics in a Lizard"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3615-0281","authenticated-orcid":false,"given":"Robin","family":"Pranter","sequence":"first","affiliation":[{"name":"Department of Biology Lund University Lund Sweden"},{"name":"Current address: Max Planck Institute for Evolutionary Biology Pl\u00f6n Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cedric","family":"Patthey","sequence":"additional","affiliation":[{"name":"Department of Diagnostic and Intervention Ume\u00e5 University Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4648-6950","authenticated-orcid":false,"given":"Nathalie","family":"Feiner","sequence":"additional","affiliation":[{"name":"Department of Biology Lund University Lund Sweden"},{"name":"Current address: Max Planck Institute for Evolutionary Biology Pl\u00f6n Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.127.3.1024"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-738-9_23"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1820320116"},{"key":"e_1_2_8_5_1","volume-title":"Developmental Biology","author":"Barresi M.","year":"2021"},{"key":"e_1_2_8_6_1","volume-title":"Handbuch der Reptilien und Amphibien Europas","author":"B\u00f6hme W.","year":"1986"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2022.06.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(86)90226-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.12.042"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0969-x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2023.10.002"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02584-9"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10012"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201646632"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.38"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511897948"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1201\/b22096"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(89)90082-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.2175"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/evo.13397"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adk9315"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4407"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad7038"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1874-1"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0497-0"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-3064-7"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01767-y"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.14.305"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60005"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089459"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.79156"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.23351"},{"key":"e_1_2_8_33_1","first-page":"1","article-title":"Early Development of The Trunk Neural Crest Cells in the Egyptian Cobra Naja H. 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