{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:02:41Z","timestamp":1790762561392,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evolution and Development"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The vertebrate\n                    <jats:italic>ahsg<\/jats:italic>\n                    (\n                    <jats:italic>alpha 2\u2010HS glycoprotein<\/jats:italic>\n                    , also coined\n                    <jats:italic>fetuin\u2010a<\/jats:italic>\n                    ) homologs are highly expressed in the liver, and their secreted protein products exert complex systemic effects, including the regulation of biomineralization of soft and skeletal tissues. Here, we report a previously uncharacterized\n                    <jats:italic>ahsg<\/jats:italic>\n                    retrocopy in the allotetraploid frog species\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    . We show that this young retrocopy was born from the\n                    <jats:italic>ahsg.L<\/jats:italic>\n                    homeologue less than 10 Mya, and landed in the S subgenome in a locus located between\n                    <jats:italic>asic2.S<\/jats:italic>\n                    and\n                    <jats:italic>smarcd2.S<\/jats:italic>\n                    . The\n                    <jats:italic>ahsg.L\u2010retrocopy<\/jats:italic>\n                    ends with a poly(A) tail, is intronless, and is flanked by target site duplications. While the\n                    <jats:italic>ahsg.L\u2010retrocopy<\/jats:italic>\n                    's ORF is devoid of frameshifts and nonsense mutations, it suffers from a short 5\u2032 deletion, eliminating the original start codon and the signal peptide. Remarkably, this truncated ORF lies in frame with an ATG codon contributed by the neighboring genomic sequence, suggesting that the\n                    <jats:italic>ahsg.L\u2010retrocopy<\/jats:italic>\n                    might potentially be expressed and translated into a protein product. Nevertheless, examination of RNA\u2010Seq and proteomic experiments respectively performed on liver and bone tissues did not provide expression evidence for the\n                    <jats:italic>ahsg.L\u2010retrocopy<\/jats:italic>\n                    . We propose that, in spite of its rescued ORF, the\n                    <jats:italic>ahsg.L\u2010retrocopy<\/jats:italic>\n                    is non\u2010functional and can be considered a young pseudogene born from recent retrotransposon activity in the\n                    <jats:italic>Xenopus laevis<\/jats:italic>\n                    lineage.\n                  <\/jats:p>","DOI":"10.1111\/ede.70060","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T05:11:01Z","timestamp":1788498661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Young\n                    <i>ahsg\/fetuin\u2010a<\/i>\n                    Inactive Retrocopy Reflects Recent Retrotransposon Activity in the\n                    <i>Xenopus laevis<\/i>\n                    Lineage"],"prefix":"10.1111","volume":"28","author":[{"given":"Marco","family":"Mundaca","sequence":"first","affiliation":[{"name":"Group for the Study of Developmental Processes (GDeP), Department of Cell Biology, School of Biological Sciences University of Concepcion Concepcion Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lefney","family":"Cumilaf","sequence":"additional","affiliation":[{"name":"Group for the Study of Developmental Processes (GDeP), Department of Cell Biology, School of Biological Sciences University of Concepcion Concepcion Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Japhet","family":"Rojas","sequence":"additional","affiliation":[{"name":"Group for the Study of Developmental Processes (GDeP), Department of Cell Biology, School of Biological Sciences University of Concepcion Concepcion Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5260-9131","authenticated-orcid":false,"given":"Roberto","family":"Amigo","sequence":"additional","affiliation":[{"name":"Group for the Study of Developmental Processes (GDeP), Department of Cell Biology, School of Biological Sciences University of Concepcion Concepcion Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Vivar","sequence":"additional","affiliation":[{"name":"Group for the Study of Developmental Processes (GDeP), Department of Cell Biology, School of Biological Sciences University of Concepcion Concepcion Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8663-2006","authenticated-orcid":false,"given":"Serge","family":"Urbach","sequence":"additional","affiliation":[{"name":"IGF Universit\u00e9 de Montpellier, CNRS, INSERM Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadija El","family":"Koulali","sequence":"additional","affiliation":[{"name":"BCM Universit\u00e9 de Montpellier, CNRS, INSERM Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5000-8735","authenticated-orcid":false,"given":"Martial","family":"S\u00e9veno","sequence":"additional","affiliation":[{"name":"BCM Universit\u00e9 de Montpellier, CNRS, INSERM Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9909-0071","authenticated-orcid":false,"given":"Camille","family":"Martinand\u2010Mari","sequence":"additional","affiliation":[{"name":"Institut des Sciences de l'Evolution de Montpellier Universit\u00e9 de Montpellier, CNRS, IRD Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1377-2515","authenticated-orcid":false,"given":"M\u00e9lanie","family":"Debiais\u2010Thibaud","sequence":"additional","affiliation":[{"name":"Institut des Sciences de l'Evolution de Montpellier Universit\u00e9 de Montpellier, CNRS, IRD Montpellier 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