{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:44:48Z","timestamp":1791225888819,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,3,15]],"date-time":"2015-03-15T00:00:00Z","timestamp":1426377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dental Traumatology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Background and aim:<\/jats:italic> Many studies have examined the nature of tissue formed in the canals of immature necrotic teeth, following revascularization in animals and humans. While speculations have been made that regeneration of the pulp tissue might take place in the canal, the tissue has been found to be cementum\u2010like, bone\u2010like, and periodontal ligament\u2010like. The purpose of this study was to histologically examine the tissue in the root canals in immature dog teeth that had been artificially infected and then revascularized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two 4\u2010 to 5\u2010month\u2010old mongrel dogs with immature teeth were used in the study. In one dog, four maxillary and four mandibular anterior teeth, and in another dog, four maxillary and five mandibular anterior teeth were used in the experiment. Pulp infection was artificially induced in the immature teeth. Revascularization was performed on all teeth by disinfecting the root canals with sodium hypochlorite irrigation and triple antibiotic intracanal dressing, completed with induction of intracanal bleeding, and sealed with an <jats:styled-content style=\"fixed-case\">MTA<\/jats:styled-content> plug. The access cavity was restored with silver amalgam. The animals were sacrificed 3\u00a0months after revascularization procedures. The revascularized teeth and surrounding periodontal tissues were removed and prepared for histological examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Besides cementum\u2010like, bone\u2010like, and periodontal ligament\u2010like tissues formed in the canals, residual remaining pulp tissue was observed in two revascularized teeth. In four teeth, ingrowth of alveolar bone into the canals was seen; presence of bone in the root canals has the potential for ankylosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within the limitation of this study, it can be concluded that residual pulp tissue can remain in the canals after revascularization procedures of immature teeth with artificially induced pulp infection. This can lead to the misinterpretation that true pulpal regeneration has occurred. Ingrowth of apical bone into the root canals undergoing revascularization can interfere with normal tooth eruption if ankylosis occurs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/edt.12169","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T05:05:58Z","timestamp":1426482358000},"page":"243-249","source":"Crossref","is-referenced-by-count":45,"title":["Histological observations of pulpal replacement tissue in immature dog teeth after revascularization of infected pulps"],"prefix":"10.1111","volume":"31","author":[{"given":"Tarek Mohamed A.","family":"Saoud","sequence":"first","affiliation":[{"name":"Faculty of Dentistry University of Benghazi Benghazi Libya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf","family":"Zaazou","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Alexandria University Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Nabil","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Alexandria University Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sybel","family":"Moussa","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Alexandria University Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanaa Mohamed","family":"Aly","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry Alexandria University Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsushi","family":"Okazaki","sequence":"additional","affiliation":[{"name":"College of Dentistry New York University New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Rosenberg","sequence":"additional","affiliation":[{"name":"College of Dentistry New York University New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis M.","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Dentistry New York University New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,15]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Stedman's medical dictionary","author":"Stedman TL","year":"2005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/etp.12040"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.03.001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.09.039"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2009.12.046"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2010.11.010"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.11.021"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.09.015"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.04.032"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.07.017"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.09.015"},{"key":"e_1_2_7_13_1","volume-title":"Cells, tissues, and disease","author":"Majno G","year":"2004"},{"key":"e_1_2_7_14_1","volume-title":"Robbins and Cotran pathologic basis of disease","author":"Kumar V","year":"2014"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.1990.tb00412.x"},{"key":"e_1_2_7_16_1","first-page":"1383","article-title":"Histologic comparison between platelet\u2010rich plasma and blood clot in regenerative endodontic treatment: an animal study","volume":"40","author":"Zhang D\u2010D","year":"2014","journal-title":"J\u00a0Endod"},{"key":"e_1_2_7_17_1","first-page":"262","article-title":"Histological evaluation of mineral trioxide aggregate and calcium hydroxide in direct pulp capping of human immature permanent teeth","volume":"21","author":"Sawicki L","year":"2008","journal-title":"Am J Dent"},{"key":"e_1_2_7_18_1","first-page":"464280","article-title":"Regenerative dentinogenesis induced by mineral trioxide aggregate: a review of biological and physicochemical points of view","author":"Okihi T","year":"2009","journal-title":"Int Dent J"},{"key":"e_1_2_7_19_1","first-page":"133","volume-title":"Seltzer and Bender's dental pulp","author":"Byers MR","year":"2012"},{"key":"e_1_2_7_20_1","first-page":"773","article-title":"Inflammatory nerve responses in the dental pulp","volume":"34","author":"Fristad I","year":"2008","journal-title":"Endod Topics"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.03.005"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509340867"}],"container-title":["Dental Traumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fedt.12169","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/edt.12169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T04:50:17Z","timestamp":1696308617000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/edt.12169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,15]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1111\/edt.12169"],"URL":"https:\/\/doi.org\/10.1111\/edt.12169","archive":["Portico"],"relation":{},"ISSN":["1600-4469","1600-9657"],"issn-type":[{"value":"1600-4469","type":"print"},{"value":"1600-9657","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,15]]}}}